DOI: 10.1073/pnas.95.5.2313. Presence and phosphorylation of transcription factors in developing dendrites [Penn]

Presence and phosphorylation of transcription factors in developing dendrites

10.1073/pnas.95.5.2313

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

In screening amplified poly(A) mRNA from hippocampal dendrites and growth cones in culture to determine candidates for local translation, we found that select transcription factor mRNAs were present. We hypothesized that synthesis of transcription factor proteins within dendrites would provide a direct signaling pathway between the distal dendrite and the nucleus resulting in modulation of gene expression important for neuronal differentiation. To evaluate this possibility, radiolabeled amplified antisense RNA was used to probe slot blots of transcription factor cDNAs as well as arrayed blots of zinc finger transcription factors. The mRNAs encoding the cAMP response element binding protein (CREB), zif 268, and one putative transcription factor were detected. We expanded upon these results showing that CREB protein is present in dendrites, that translation of CREB mRNA in isolated dendrites is feasible and that CREB protein found in dendrites can interact with the cis-acting cyclic AMP reponse element DNA sequence by using an in situ Southwestern assay. Further, CREB protein in dendrites is not transported to this site from the cell body because fluorescently tagged CREB microperfused into the soma did not diffuse into the dendrites. In addition, CREB protein microperfused into dendrites was rapidly transported to the nucleus, its likely site of bioactivity. Lastly, by using the isolated dendrite system we show that phosphorylation of Ser-133 on CREB protein can occur in isolated dendrites independent of the nucleus. These data provide a regulatory pathway in which transcription factors synthesized and posttranslationally modified in dendrites directly alter gene expression bypassing the integration of signal transduction pathways that converge on the nucleus.

Bibliography

Crino, P., Khodakhah, K., Becker, K., Ginsberg, S., Hemby, S., & Eberwine, J. (1998). Presence and phosphorylation of transcription factors in developing dendrites. Proceedings of the National Academy of Sciences, 95(5), 2313â2318.

Authors 6

Peter Crino (first)
Kamran Khodakhah (additional)
Kevin Becker (additional)
Stephen Ginsberg (additional)
Scott Hemby (additional)
James Eberwine (additional)

References 27 Referenced 132

Dates

Type	When
Created	23 years ago (July 26, 2002, 10:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:34 p.m.)
Indexed	1 month, 2 weeks ago (July 7, 2025, 1:45 a.m.)
Issued	27 years, 5 months ago (March 3, 1998)
Published	27 years, 5 months ago (March 3, 1998)
Published Online	27 years, 5 months ago (March 3, 1998)
Published Print	27 years, 5 months ago (March 3, 1998)

Funders 0

None

BibTeX

@article{Crino_1998, title={Presence and phosphorylation of transcription factors in developing dendrites}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.5.2313}, DOI={10.1073/pnas.95.5.2313}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Crino, Peter and Khodakhah, Kamran and Becker, Kevin and Ginsberg, Stephen and Hemby, Scott and Eberwine, James}, year={1998}, month=mar, pages={2313–2318} }

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