DOI: 10.1073/pnas.95.5.2267. Protein hydration in solution: Experimental observation by x-ray and neutron scattering

Protein hydration in solution: Experimental observation by x-ray and neutron scattering

10.1073/pnas.95.5.2267

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The structure of the protein–solvent interface is the subject of controversy in theoretical studies and requires direct experimental characterization. Three proteins with known atomic resolution crystal structure (lysozyme, Escherichia coli thioredoxin reductase, and protein R1 of E. coli ribonucleotide reductase) were investigated in parallel by x-ray and neutron scattering in H 2 O and D 2 O solutions. The analysis of the protein–solvent interface is based on the significantly different contrasts for the protein and for the hydration shell. The results point to the existence of a first hydration shell with an average density ≈10% larger than that of the bulk solvent in the conditions studied. Comparisons with the results of other studies suggest that this may be a general property of aqueous interfaces.

Bibliography

Svergun, D. I., Richard, S., Koch, M. H. J., Sayers, Z., Kuprin, S., & Zaccai, G. (1998). Protein hydration in solution: Experimental observation by x-ray and neutron scattering. Proceedings of the National Academy of Sciences, 95(5), 2267â2272.

Authors 6

D. I. Svergun (first)
S. Richard (additional)
M. H. J. Koch (additional)
Z. Sayers (additional)
S. Kuprin (additional)
G. Zaccai (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:32 p.m.)
Indexed	2 days, 14 hours ago (Aug. 30, 2025, 12:20 p.m.)
Issued	27 years, 5 months ago (March 3, 1998)
Published	27 years, 5 months ago (March 3, 1998)
Published Online	27 years, 5 months ago (March 3, 1998)
Published Print	27 years, 5 months ago (March 3, 1998)

Funders 0

None

BibTeX

@article{Svergun_1998, title={Protein hydration in solution: Experimental observation by x-ray and neutron scattering}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.5.2267}, DOI={10.1073/pnas.95.5.2267}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Svergun, D. I. and Richard, S. and Koch, M. H. J. and Sayers, Z. and Kuprin, S. and Zaccai, G.}, year={1998}, month=mar, pages={2267–2272} }

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