DOI: 10.1073/pnas.95.4.1460. A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics

A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics

10.1073/pnas.95.4.1460

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A unified view of polymer, dumbbell, and oligonucleotide nearest-neighbor (NN) thermodynamics is presented. DNA NN ΔG° 37 parameters from seven laboratories are presented in the same format so that careful comparisons can be made. The seven studies used data from natural polymers, synthetic polymers, oligonucleotide dumbbells, and oligonucleotide duplexes to derive NN parameters; used different methods of data analysis; used different salt concentrations; and presented the NN thermodynamics in different formats. As a result of these differences, there has been much confusion regarding the NN thermodynamics of DNA polymers and oligomers. Herein I show that six of the studies are actually in remarkable agreement with one another and explanations are provided in cases where discrepancies remain. Further, a single set of parameters, derived from 108 oligonucleotide duplexes, adequately describes polymer and oligomer thermodynamics. Empirical salt dependencies are also derived for oligonucleotides and polymers.

Bibliography

SantaLucia, J. (1998). A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighborâthermodynamics. Proceedings of the National Academy of Sciences, 95(4), 1460â1465.

Authors 1

John SantaLucia (first)

References 42 Referenced 2,292

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:51 p.m.)
Indexed	2 weeks, 6 days ago (Aug. 7, 2025, 4:51 a.m.)
Issued	27 years, 6 months ago (Feb. 17, 1998)
Published	27 years, 6 months ago (Feb. 17, 1998)
Published Online	27 years, 6 months ago (Feb. 17, 1998)
Published Print	27 years, 6 months ago (Feb. 17, 1998)

Funders 0

None

BibTeX

@article{SantaLucia_1998, title={A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.4.1460}, DOI={10.1073/pnas.95.4.1460}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={SantaLucia, John}, year={1998}, month=feb, pages={1460–1465} }

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