Homology and functional similarity of an hrp -linked pathogenicity locus, dspEF , of Erwinia amylovora and the avirulence locus avrE of Pseudomonas syringae pathovar tomato
10.1073/pnas.95.3.1325
Crossref journal-article
Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences (341)
Abstract

The “disease-specific” ( dsp ) region next to the hrp gene cluster of Erwinia amylovora is required for pathogenicity but not for elicitation of the hypersensitive reaction. A 6.6-kb apparent operon, dspEF , was found responsible for this phenotype. The operon contains genes dspE and dspF and is positively regulated by hrpL . A blast search revealed similarity in the dspE gene to a partial sequence of the avrE locus of Pseudomonas syringae pathovar tomato. The entire avrE locus was sequenced. Homologs of dspE and dspF were found in juxtaposed operons and were designated avrE and avrF . Introduced on a plasmid, the dspEF locus rendered P. syringae pv. glycinea race 4 avirulent on soybean. An E. amylovora dspE mutant, however, elicited a hypersensitive reaction in soybean. The avrE locus in trans restored pathogenicity to dspE strains of E. amylovora , although restored strains were low in virulence. DspE and AvrE are large (198 kDa and 195 kDa) and hydrophilic. DspF and AvrF are small (16 kDa and 14 kDa) and acidic with predicted amphipathic α helices in their C termini; they resemble chaperones for virulence factors secreted by type III secretion systems of animal pathogens.

Bibliography

Bogdanove, A. J., Kim, J. F., Wei, Z., Kolchinsky, P., Charkowski, A. O., Conlin, A. K., Collmer, A., & Beer, S. V. (1998). Homology and functional similarity of an hrp -linked pathogenicity locus, dspEF , of Erwinia amylovora and the avirulence locus avrE of Pseudomonas syringae pathovar tomato. Proceedings of the National Academy of Sciences, 95(3), 1325–1330.

Authors 8
  1. Adam J. Bogdanove (first)
  2. Jihyun F. Kim (additional)
  3. Zhongmin Wei (additional)
  4. Peter Kolchinsky (additional)
  5. Amy O. Charkowski (additional)
  6. Alison K. Conlin (additional)
  7. Alan Collmer (additional)
  8. Steven V. Beer (additional)
References 51 Referenced 154
  1. Z-M Wei, S V Beer J Bacteriol 177, 6201–6210 (1995). (10.1128/jb.177.21.6201-6210.1995) / J Bacteriol by Wei Z-M (1995)
  2. A J Bogdanove, Z-M Wei, L Zhao, S V Beer J Bacteriol 178, 1720–1730 (1996). (10.1128/jb.178.6.1720-1730.1996) / J Bacteriol by Bogdanove A J (1996)
  3. J-H Kim, Z-M Wei, S V Beer J Bacteriol 179, 1690–1697 (1997). (10.1128/jb.179.5.1690-1697.1997) / J Bacteriol by Kim J-H (1997)
  4. Z M Wei, R J Laby, C H Zumoff, D W Bauer, S Y He, A Collmer, S V Beer Science 257, 85–88 (1992). (10.1126/science.1621099) / Science by Wei Z M (1992)
  5. J F Kim Ph.D. dissertation (Cornell University, Ithaca, NY, 1997). / Ph.D. dissertation by Kim J F (1997)
  6. J R Alfano, A Collmer Plant Cell 8, 1683–1698 (1996). (10.2307/3870222) / Plant Cell by Alfano J R (1996)
  7. J L Dangl Bacterial Pathogenesis of Plants and Animals: Molecular and Cellular Mechanisms (Curr. Topics Microbiol. Immunol.), ed J L Dangl (Springer, Berlin) 192, 99–118 (1994). (10.1007/978-3-642-78624-2_5) / Bacterial Pathogenesis of Plants and Animals: Molecular and Cellular Mechanisms (Curr. Topics Microbiol. Immunol.) by Dangl J L (1994)
  8. D Y Kobayashi, S J Tamaki, N T Keen Proc Natl Acad Sci USA 86, 157–161 (1989). (10.1073/pnas.86.1.157) / Proc Natl Acad Sci USA by Kobayashi D Y (1989)
  9. M C Whalen, R E Stall, B J Staskawicz Proc Natl Acad Sci USA 85, 6743–6747 (1988). (10.1073/pnas.85.18.6743) / Proc Natl Acad Sci USA by Whalen M C (1988)
  10. S Swarup, Y Yang, M T Kingsley, D W Gabriel Mol Plant–Microbe Interact 5, 204–213 (1992). (10.1094/MPMI-5-204) / Mol Plant–Microbe Interact by Swarup S (1992)
  11. J Mansfield, C Jenner, R Hockenhull, M A Bennett, R Stewart Mol Plant–Microbe Interact 7, 726–739 (1994). (10.1094/MPMI-7-0726) / Mol Plant–Microbe Interact by Mansfield J (1994)
  12. B Kearney, B J Staskawicz Nature (London) 346, 385–386 (1990). (10.1038/346385a0) / Nature (London) by Kearney B (1990)
  13. S Swarup, R De Feyter, R H Brlansky, D N Gabriel Phytopathology 81, 802–808 (1991). (10.1094/Phyto-81-802) / Phytopathology by Swarup S (1991)
  14. R D De Feyter, Y Yang, D W Gabriel Mol Plant–Microbe Interact 6, 225–237 (1993). (10.1094/MPMI-6-225) / Mol Plant–Microbe Interact by De Feyter R D (1993)
  15. J M Lorang, H Shen, D Kobayashi, D Cooksey, N T Keen Mol Plant–Microbe Interact 7, 508–515 (1994). (10.1094/MPMI-7-0508) / Mol Plant–Microbe Interact by Lorang J M (1994)
  16. C Ritter, J L Dangl Mol Plant–Microbe Interact 8, 444–453 (1995). (10.1094/MPMI-8-0444) / Mol Plant–Microbe Interact by Ritter C (1995)
  17. J M Lorang, N T Keen Mol Plant–Microbe Interact 8, 49–57 (1995). (10.1094/MPMI-8-0049) / Mol Plant–Microbe Interact by Lorang J M (1995)
  18. Y Xiao, S W Hutcheson J Bacteriol 176, 3089–3091 (1994). (10.1128/jb.176.10.3089-3091.1994) / J Bacteriol by Xiao Y (1994)
  19. A M Barny, M H Guinebretiere, B Marcais, E Coissac, J P Paulin, J Laurent Mol Microbiol 4, 777–786 (1990). (10.1111/j.1365-2958.1990.tb00648.x) / Mol Microbiol by Barny A M (1990)
  20. J Sambrook, E F Fritsch, T E Maniatis Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Lab. Press, Cold Spring Harbor, NY, 1989). / Molecular Cloning: A Laboratory Manual by Sambrook J (1989)
  21. D W Bauer, A Collmer Mol Plant–Microbe Interact 10, 369–379 (1997). (10.1094/MPMI.1997.10.3.369) / Mol Plant–Microbe Interact by Bauer D W (1997)
  22. D W Bauer Ph.D. dissertation (Cornell University, Ithaca, NY, 1990). / Ph.D. dissertation by Bauer D W (1990)
  23. D Figurski, D R Helinski Proc Natl Acad Sci USA 76, 1648–1652 (1979). (10.1073/pnas.76.4.1648) / Proc Natl Acad Sci USA by Figurski D (1979)
  24. S V Beer, D W Bauer, X H Jiang, R J Laby, B J Sneath, Z M Wei, D A Wilcox, C H Zumoff Advances in Molecular Genetics of Plant–Microbe Interactions, eds H Hennecke, D P S Verma (Kluwer, Dordrecht, The Netherlands), pp. 53–60 (1991). (10.1007/978-94-015-7934-6_8) / Advances in Molecular Genetics of Plant–Microbe Interactions by Beer S V (1991)
  25. S F Altschul, D J Lipman Proc Nat Acad Sci USA 87, 5509–5513 (1990). (10.1073/pnas.87.14.5509) / Proc Nat Acad Sci USA by Altschul S F (1990)
  26. G D Duffaud, P E March, M Inouye Methods Enzymol 153, 492–507 (1987). (10.1016/0076-6879(87)53074-9) / Methods Enzymol by Duffaud G D (1987)
  27. K Kaniga, I Delor, G R Cornelis Gene 109, 137–142 (1991). (10.1016/0378-1119(91)90599-7) / Gene by Kaniga K (1991)
  28. S V Beer Methods in Phytobacteriology, eds Z Klement, K Rudolph, D C Sands (Akadémiai Kiadó, Budapest), pp. 373–374 (1990). / Methods in Phytobacteriology by Beer S V (1990)
  29. H S Aldwinckle, J L Preczewski Phytopathology 66, 1439–1444 (1976). (10.1094/Phyto-66-1439) / Phytopathology by Aldwinckle H S (1976)
  30. D W Bauer, S V Beer Mol Plant–Microbe Interact 4, 493–499 (1991). (10.1094/MPMI-4-493) / Mol Plant–Microbe Interact by Bauer D W (1991)
  31. T V Huynh, D Dahlbeck, B J Staskawicz Science 345, 1374–1377 (1989). (10.1126/science.2781284) / Science by Huynh T V (1989)
  32. R A Jefferson Plant Mol Biol Rep 5, 387–405 (1987). (10.1007/BF02667740) / Plant Mol Biol Rep by Jefferson R A (1987)
  33. J H Miller A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria (Cold Spring Harbor Lab. Press, Plainview, NY, 1992). / A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria by Miller J H (1992)
  34. E M Steinberger, S V Beer Mol Plant–Microbe Interact 1, 135–144 (1988). (10.1094/MPMI-1-135) / Mol Plant–Microbe Interact by Steinberger E M (1988)
  35. A L Caldwell, P A Gulig J Bacteriol 173, 7176–7185 (1991). (10.1128/jb.173.22.7176-7185.1991) / J Bacteriol by Caldwell A L (1991)
  36. P Wattiau, S Woestyn, G R Cornelis Mol Microbiol 20, 255–262 (1996). (10.1111/j.1365-2958.1996.tb02614.x) / Mol Microbiol by Wattiau P (1996)
  37. S F Altschul, W Gish, W Miller, E W Myers, D J Lipman J Mol Biol 215, 403–410 (1990). (10.1016/S0022-2836(05)80360-2) / J Mol Biol by Altschul S F (1990)
  38. M Saraste, P R Sibbald, A Wittinghofer Trends Biochem Sci 15, 430–434 (1990). (10.1016/0968-0004(90)90281-F) / Trends Biochem Sci by Saraste M (1990)
  39. M Labes, A Puehler, R Simon Gene 89, 37–46 (1990). (10.1016/0378-1119(90)90203-4) / Gene by Labes M (1990)
  40. M A Barny Eur J Plant Pathol 101, 333–340 (1995). (10.1007/BF01874789) / Eur J Plant Pathol by Barny M A (1995)
  41. A Collmer, J R Alfano, D W Bauer, G M Preston, A O Loniello, A Conlin, J H Ham, H-C Huang, S Gopalan, S Y He Advances in Molecular Genetics of Plant–Microbe Interactions, eds G Stacey, B Mullin, P M Gresshoff (APS Press, St. Paul) 4, 159–164 (1996). / Advances in Molecular Genetics of Plant–Microbe Interactions by Collmer A (1996)
  42. G Van den Ackerveken, U Bonas Trends Microbiol 5, 394–398 (1997). (10.1016/S0966-842X(97)01124-4) / Trends Microbiol by Van den Ackerveken G (1997)
  43. J E Galán, J B Bliska Annu Rev Cell Dev Biol 12, 221–255 (1996). (10.1146/annurev.cellbio.12.1.221) / Annu Rev Cell Dev Biol by Galán J E (1996)
  44. M C Whalen, J F Wang, F M Carland, M E Heiskell, D Dahlbeck, G V Minsavage, J B Jones, J W Scott, R E Stall, B J Staskawicz Mol Plant–Microbe Interact 6, 616–627 (1993). (10.1094/MPMI-6-616) / Mol Plant–Microbe Interact by Whalen M C (1993)
  45. J L Norelli, H S Aldwinckle, S V Beer Phytopathology 74, 136–139 (1984). (10.1094/Phyto-74-136) / Phytopathology by Norelli J L (1984)
  46. M P Starr, C Cardona, D Folsom Phytopathology 41, 915–919 (1951). / Phytopathology by Starr M P (1951)
  47. S V Beer, Z M Wei, R J Laby, S Y He, D W Bauer, A Collmer, C Zumoff Advances in Molecular Genetics of Plant–Microbe Interactions, eds E W Nester, D P S Verma (Kluwer, Dordrecht, The Netherlands), pp. 281–286 (1993). (10.1007/978-94-017-0651-3_31) / Advances in Molecular Genetics of Plant–Microbe Interactions by Beer S V (1993)
  48. E B Williams, J Kuc Annu Rev Phytopathol 7, 223–246 (1969). (10.1146/annurev.py.07.090169.001255) / Annu Rev Phytopathol by Williams E B (1969)
  49. J L Norelli, H S Aldwinckle, L Destéfano Beltrán, J M Jaynes Euphytica 77, 123–128 (1994). (10.1007/BF02551474) / Euphytica by Norelli J L (1994)
  50. L Parisi, Y Lespinasse, J Guillaumes, J Krueger Phytopathology 83, 533–537 (1993). (10.1094/Phyto-83-533) / Phytopathology by Parisi L (1993)
  51. S Gaudriault, L Malandrin, J-P Paulin, M-A Barny Mol Microbiol 26, 1057–1069 (1997). (10.1046/j.1365-2958.1997.6442015.x) / Mol Microbiol by Gaudriault S (1997)
Dates
Type When
Created 23 years ago (July 26, 2002, 10:40 a.m.)
Deposited 3 years, 2 months ago (June 6, 2022, 10:45 p.m.)
Indexed 1 week ago (Aug. 19, 2025, 6:26 a.m.)
Issued 27 years, 6 months ago (Feb. 3, 1998)
Published 27 years, 6 months ago (Feb. 3, 1998)
Published Online 27 years, 6 months ago (Feb. 3, 1998)
Published Print 27 years, 6 months ago (Feb. 3, 1998)
Funders 0

None

@article{Bogdanove_1998, title={Homology and functional similarity of an hrp -linked pathogenicity locus, dspEF , of Erwinia amylovora and the avirulence locus avrE of Pseudomonas syringae pathovar tomato}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.3.1325}, DOI={10.1073/pnas.95.3.1325}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Bogdanove, Adam J. and Kim, Jihyun F. and Wei, Zhongmin and Kolchinsky, Peter and Charkowski, Amy O. and Conlin, Alison K. and Collmer, Alan and Beer, Steven V.}, year={1998}, month=feb, pages={1325–1330} }