DOI: 10.1073/pnas.95.3.1172. HLA class I-specific inhibitory receptors in human T lymphocytes: Interleukin 15-induced expression of CD94/NKG2A in superantigen- or alloantigen-activated CD8<sup>+</sup> T cells

HLA class I-specific inhibitory receptors in human T lymphocytes: Interleukin 15-induced expression of CD94/NKG2A in superantigen- or alloantigen-activated CD8+ T cells

10.1073/pnas.95.3.1172

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A fraction of human T lymphocytes, predominantly CD8+, express receptors for HLA class I molecules typical of natural killer cells (natural killer receptors or NKRs) that inhibit T cell receptor-mediated functions. Herein, we analyzed possible mechanism(s) leading to the expression of NKRs by T cells responding to superantigens or allogeneic cellsin vitro. We show that, in the presence of interleukin 15 (IL-15), T cells (depleted of NKR+cells) responding to toxic shock syndrome toxin 1de novoexpress CD94, a molecule that is part of a heterodimeric NKR with a broad specificity for different HLA class I alleles. Maximal CD94 expression occurred when IL-15 was added shortly after the cells were placed into culture, and CD94 expression started 4–6 days after addition of IL-15. Although both CD4+and CD8+cells expressed CD94, the simultaneous expression of NKG2A (i.e., the other component of the CD94/NKG2A inhibitory NKR) was confined to CD8+cells. Similar data were obtained in T cell populations activated in mixed lymphocyte cultures in the presence of IL-15. The expression of CD94/NKG2A led to an impairment of allo-specific cytolytic activity by mixed lymphocyte culture-derived T cell populations or clones. Remarkably, cytolysis could be restored by the addition of anti-CD94 mAb, i.e., by masking the inhibitory NKRs.

Bibliography

Mingari, M. C., Ponte, M., Bertone, S., Schiavetti, F., Vitale, C., Bellomo, R., Moretta, A., & Moretta, L. (1998). HLA class I-specific inhibitory receptors in human T lymphocytes: Interleukin 15-induced expression of CD94/NKG2A in superantigen- or alloantigen-activated CD8+âTâcells. Proceedings of the National Academy of Sciences, 95(3), 1172â1177.

Authors 8

Maria Cristina Mingari (first)
Marco Ponte (additional)
Stefania Bertone (additional)
Francesca Schiavetti (additional)
Chiara Vitale (additional)
Rosa Bellomo (additional)
Alessandro Moretta (additional)
Lorenzo Moretta (additional)

References 31 Referenced 195

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J Aramburu, M A Balboa, A Ramirez, A Silva, A Acevedo, F Sanchez-Madrid, M O de Landàzuri, M López-Botet J Immunol 44, 3238–3247 (1990). (10.4049/jimmunol.144.8.3238) / J Immunol by Aramburu J (1990)
10.1084/jem.180.2.545
10.1002/eji.1830261032
S Lazetic, C Chang, J P Houchins, L L Lanier, J H Phillips J Immunol 157, 4741–4745 (1996). (10.4049/jimmunol.157.11.4741) / J Immunol by Lazetic S (1996)
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10.1146/annurev.iy.12.040194.001303
10.1093/intimm/9.4.485
10.1073/pnas.94.15.8088

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:32 a.m.)
Deposited	2 years, 4 months ago (April 22, 2023, 8:11 a.m.)
Indexed	2 weeks, 6 days ago (Aug. 6, 2025, 9:50 a.m.)
Issued	27 years, 6 months ago (Feb. 3, 1998)
Published	27 years, 6 months ago (Feb. 3, 1998)
Published Online	27 years, 6 months ago (Feb. 3, 1998)
Published Print	27 years, 6 months ago (Feb. 3, 1998)

Funders 0

None

BibTeX

@article{Mingari_1998, title={HLA class I-specific inhibitory receptors in human T lymphocytes: Interleukin 15-induced expression of CD94/NKG2A in superantigen- or alloantigen-activated CD8+ T cells}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.3.1172}, DOI={10.1073/pnas.95.3.1172}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Mingari, Maria Cristina and Ponte, Marco and Bertone, Stefania and Schiavetti, Francesca and Vitale, Chiara and Bellomo, Rosa and Moretta, Alessandro and Moretta, Lorenzo}, year={1998}, month=feb, pages={1172–1177} }

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