DOI: 10.1073/pnas.95.3.1136. Targeted disruption of mouse centromere protein C gene leads to mitotic disarray and early embryo death

Targeted disruption of mouse centromere protein C gene leads to mitotic disarray and early embryo death

10.1073/pnas.95.3.1136

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Centromere protein C (CENPC) is a key protein that has been localized to the inner kinetochore plate of active mammalian centromeres. Using gene targeting techniques, we have disrupted the mouseCenpcgene and shown that the gene is essential for normal mouse embryonic development. Heterozygous mice carrying one functional copy of the gene are healthy and fertile, whereas homozygous embryos fail to thrive. In these embryos, mitotic arrest and gross morphological degeneration become apparent as early as the morula stage of development. The degenerating embryos demonstrate highly irregular cell and nuclear morphologies, including the presence of a large number of micronuclei. Mitotic chromosomes of these embryos display a scattered and often highly condensed configuration and do not segregate in an ordered fashion. These results describing the phenotype of the mutant mouse embryos indicate that CENPC has a direct role in the mitotic progression from metaphase to anaphase.

Bibliography

Kalitsis, P., Fowler, K. J., Earle, E., Hill, J., & Choo, K. H. A. (1998). Targeted disruption of mouse centromere protein C gene leads to mitotic disarray and early embryoâdeath. Proceedings of the National Academy of Sciences, 95(3), 1136â1141.

Authors 5

Paul Kalitsis (first)
Kerry J. Fowler (additional)
Elizabeth Earle (additional)
Joanne Hill (additional)
K. H. Andy Choo (additional)

References 40 Referenced 115

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:35 a.m.)
Deposited	1 year, 8 months ago (Jan. 3, 2024, 11:50 p.m.)
Indexed	4 weeks, 1 day ago (Aug. 7, 2025, 4:36 p.m.)
Issued	27 years, 7 months ago (Feb. 3, 1998)
Published	27 years, 7 months ago (Feb. 3, 1998)
Published Online	27 years, 7 months ago (Feb. 3, 1998)
Published Print	27 years, 7 months ago (Feb. 3, 1998)

Funders 0

None

BibTeX

@article{Kalitsis_1998, title={Targeted disruption of mouse centromere protein C gene leads to mitotic disarray and early embryo death}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.3.1136}, DOI={10.1073/pnas.95.3.1136}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kalitsis, Paul and Fowler, Kerry J. and Earle, Elizabeth and Hill, Joanne and Choo, K. H. Andy}, year={1998}, month=feb, pages={1136–1141} }

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