DOI: 10.1073/pnas.95.26.15315. Protein fluctuations are sensed by stimulated infrared echoes of the vibrations of carbon monoxide and azide probes [Penn]

Protein fluctuations are sensed by stimulated infrared echoes of the vibrations of carbon monoxide and azide probes

10.1073/pnas.95.26.15315

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The correlation functions of the fluctuations of vibrational frequencies of azide ions and carbon monoxide in proteins are determined directly from stimulated photon echoes generated with femtosecond infrared pulses. The asymmetric stretching vibration of azide bound to carbonic anhydrase II exhibits a pronounced evolution of its vibrational frequency distribution on the time scale of a few picoseconds, which is attributed to modifications of the ligand structure through interactions with the nearby Thr-199. When azide is bound in hemoglobin, a more complex evolution of the protein structure is required to interchange the different ligand configurations, as evidenced by the much slower relaxation of the frequency distribution in this case. The time evolution of the distribution of frequencies of carbon monoxide bound in hemoglobin occurs on the ≈10-ps time scale and is very nonexponential. The correlation functions of the frequency fluctuations determine the evolution of the protein structure local to the probe and the extent to which the probe can navigate those parts of the energy landscape where the structural configurations are able to modify the local potential energy function of the probe.

Bibliography

Lim, M., Hamm, P., & Hochstrasser, R. M. (1998). Protein fluctuations are sensed by stimulated infrared echoes of the vibrations of carbon monoxide and azide probes. Proceedings of the National Academy of Sciences, 95(26), 15315â15320.

Authors 3

Manho Lim (first)
Peter Hamm (additional)
Robin M. Hochstrasser (additional)

References 48 Referenced 129

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:35 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:12 p.m.)
Indexed	1 week, 6 days ago (Aug. 21, 2025, 1:26 p.m.)
Issued	26 years, 8 months ago (Dec. 22, 1998)
Published	26 years, 8 months ago (Dec. 22, 1998)
Published Online	26 years, 8 months ago (Dec. 22, 1998)
Published Print	26 years, 8 months ago (Dec. 22, 1998)

Funders 0

None

BibTeX

@article{Lim_1998, title={Protein fluctuations are sensed by stimulated infrared echoes of the vibrations of carbon monoxide and azide probes}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.26.15315}, DOI={10.1073/pnas.95.26.15315}, number={26}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Lim, Manho and Hamm, Peter and Hochstrasser, Robin M.}, year={1998}, month=dec, pages={15315–15320} }

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