DOI: 10.1073/pnas.95.26.15281. RNA polymerase subunit RPB5 plays a role in transcriptional activation

RNA polymerase subunit RPB5 plays a role in transcriptional activation

10.1073/pnas.95.26.15281

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A mutation in RPB5 ( rpb5–9 ) , an essential RNA polymerase subunit assembled into RNA polymerases I, II, and III, revealed a role for this subunit in transcriptional activation. Activation by GAL4-VP16 was impaired upon in vitro transcription with mutant whole-cell extracts. In vivo experiments using inducible reporter plasmids and Northern analysis support the in vitro data and demonstrate that RPB5 influences activation at some, but not all, promoters. Remarkably, this mutation maps to a conserved region of human RPB5 implicated by others to play a role in activation. Chimeric human-yeast RPB5 containing this conserved region now can function in place of its yeast counterpart. The defects noted with rpb5–9 are similar to those seen in truncation mutants of the RPB1-carboxyl terminal domain (CTD). We demonstrate that RPB5 and the RPB1-CTD have overlapping roles in activation because the double mutant is synthetically lethal and has exacerbated activation defects at the GAL1/10 promoter. These studies demonstrate that there are multiple activation targets in RNA polymerase II and that RPB5 and the CTD have similar roles in activation.

Bibliography

Miyao, T., & Woychik, N. A. (1998). RNA polymerase subunit RPB5 plays a role in transcriptional activation. Proceedings of the National Academy of Sciences, 95(26), 15281â15286.

Authors 2

Takenori Miyao (first)
Nancy A. Woychik (additional)

References 51 Referenced 46

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:13 p.m.)
Indexed	2 months ago (July 3, 2025, 10:47 a.m.)
Issued	26 years, 8 months ago (Dec. 22, 1998)
Published	26 years, 8 months ago (Dec. 22, 1998)
Published Online	26 years, 8 months ago (Dec. 22, 1998)
Published Print	26 years, 8 months ago (Dec. 22, 1998)

Funders 0

None

BibTeX

@article{Miyao_1998, title={RNA polymerase subunit RPB5 plays a role in transcriptional activation}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.26.15281}, DOI={10.1073/pnas.95.26.15281}, number={26}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Miyao, Takenori and Woychik, Nancy A.}, year={1998}, month=dec, pages={15281–15286} }

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