DOI: 10.1073/pnas.95.25.14646. Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in <i>Escherichia coli</i>

Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli

10.1073/pnas.95.25.14646

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Assembly of several inner membrane proteins—leader peptidase (Lep), a Lep derivative (Lep-inv) that inserts with an inverted topology compared with the wild-type protein, the phage M13 procoat protein, and a procoat derivative (H1-procoat) with the hydrophobic core of the signal peptide replaced by a stretch from the first transmembrane segment in Lep—has been studied in vitro and in Escherichia coli strains that are conditional for the expression of either the 54 homologue (Ffh) or 4.5S RNA, which are the two components of the E. coli signal recognition particle (SRP), or SecE, an essential core component of the E. coli preprotein translocase. Membrane insertion has also been tested in a SecB null strain. Lep, Lep-inv, and H1-procoat require SRP for correct assembly into the inner membrane; in contrast, we find that wild-type procoat does not. Lep and, surprisingly, Lep-inv and H1-procoat fail to insert properly when SecE is depleted, whereas insertion of wild-type procoat is unaffected under these conditions. None of the proteins depend on SecB for assembly. These observations indicate that inner membrane proteins can assemble either by a mechanism in which SRP delivers the protein at the preprotein translocase or by what appears to be a direct integration into the lipid bilayer. The observed change in assembly mechanism when the hydrophobicity of the procoat signal peptide is increased demonstrates that the assembly of an inner membrane protein can be rerouted between different pathways.

Bibliography

de Gier, J.-W. L., Scotti, P. A., SÃ¤Ã¤f, A., Valent, Q. A., Kuhn, A., Luirink, J., & von Heijne, G. (1998). Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli. Proceedings of the National Academy of Sciences, 95(25), 14646â14651.

Authors 7

Jan-Willem L. de Gier (first)
Pier A. Scotti (additional)
Annika Sääf (additional)
Quido A. Valent (additional)
Andreas Kuhn (additional)
Joen Luirink (additional)
Gunnar von Heijne (additional)

References 54 Referenced 102

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:59 p.m.)
Indexed	1 year, 1 month ago (July 23, 2024, 9:17 a.m.)
Issued	26 years, 8 months ago (Dec. 8, 1998)
Published	26 years, 8 months ago (Dec. 8, 1998)
Published Online	26 years, 8 months ago (Dec. 8, 1998)
Published Print	26 years, 8 months ago (Dec. 8, 1998)

Funders 0

None

BibTeX

@article{de_Gier_1998, title={Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.25.14646}, DOI={10.1073/pnas.95.25.14646}, number={25}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={de Gier, Jan-Willem L. and Scotti, Pier A. and Sääf, Annika and Valent, Quido A. and Kuhn, Andreas and Luirink, Joen and von Heijne, Gunnar}, year={1998}, month=dec, pages={14646–14651} }

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