DOI: 10.1073/pnas.95.24.14570. Mode of action of the <i>COR15a</i> gene on the freezing tolerance of <i>Arabidopsis thaliana</i>

Mode of action of the COR15a gene on the freezing tolerance of Arabidopsis thaliana

10.1073/pnas.95.24.14570

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Constitutive expression of the co ld- r egulated COR15a gene of Arabidopsis thaliana results in a significant increase in the survival of isolated protoplasts frozen over the range of −4.5 to −7°C. The increased freezing tolerance is the result of a decreased incidence of freeze-induced lamellar-to-hexagonal II phase transitions that occur in regions where the plasma membrane is brought into close apposition with the chloroplast envelope as a result of freeze-induced dehydration. Moreover, the mature polypeptide encoded by this gene, COR15am, increases the lamellar-to-hexagonal II phase transition temperature of dioleoylphosphatidylethanolamine and promotes formation of the lamellar phase in a lipid mixture composed of the major lipid species that comprise the chloroplast envelope. We propose that COR15am, which is located in the chloroplast stroma, defers freeze-induced formation of the hexagonal II phase to lower temperatures (lower hydrations) by altering the intrinsic curvature of the inner membrane of the chloroplast envelope.

Bibliography

Steponkus, P. L., Uemura, M., Joseph, R. A., Gilmour, S. J., & Thomashow, M. F. (1998). Mode of action of the COR15a gene on the freezing tolerance of Arabidopsis thaliana. Proceedings of the National Academy of Sciences, 95(24), 14570â14575.

Authors 5

Peter L. Steponkus (first)
Matsuo Uemura (additional)
Raymond A. Joseph (additional)
Sarah J. Gilmour (additional)
Michael F. Thomashow (additional)

References 38 Referenced 318

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10.1016/S0065-2660(08)60525-8
M F Thomashow Advances in Low-Temperature Biology, ed P L Steponkus (JAI Press, London) 2, 183–210 (1993). / Advances in Low-Temperature Biology by Thomashow M F (1993)
10.1104/pp.99.2.519
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10.1104/pp.84.3.872
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M F Thomashow, S J Gilmour, R Hajela, D Horvath, C Lin, W Guo Horticultural Biotechnology, eds A B Bennett, S D O’Neill (Wiley-Liss, New York), pp. 305–314 (1990). / Horticultural Biotechnology by Thomashow M F (1990)
10.1016/0005-2795(75)90048-3
D K Hincha, F Sieg, I Bakaltcheva, H Köth, J M Schmitt Advances in Low-Temperature Biology, ed P L Steponkus (JAI Press, London) 3, 141–183 (1996). (10.1016/S1873-9792(96)80006-9) / Advances in Low-Temperature Biology by Hincha D K (1996)
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P L Steponkus Endocytosis, Exocytosis, and Vesicle Traffic in Plants, eds C R Hawes, J O D Coleman, D E Evans (Soc. Exp. Biol. Sem., Cambridge, , Series 45, pp. 103–128. (1991). / Endocytosis, Exocytosis, and Vesicle Traffic in Plants by Steponkus P L (1991)
P L Steponkus, M Uemura, M S Webb Advances in Low-Temperature Biology, ed P L Steponkus (JAI Press, London) 2, 211–312 (1993). / Advances in Low-Temperature Biology by Steponkus P L (1993)
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P L Steponkus, M Uemura, M S Webb Permeability and Stability of Lipid Bilayers, eds E A Disalvo, S A Simon (CRC Press, Boca Raton, FL), pp. 77–104 (1995). / Permeability and Stability of Lipid Bilayers by Steponkus P L (1995)
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10.1016/S0021-9258(17)44113-5
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M Uemura, P L Steponkus Plant Cold Hardiness Molecular Biology, Biochemistry, and Physiology, eds P H Li, T T Chen (Plenum, New York), pp. 171–179 (1997). / Plant Cold Hardiness Molecular Biology, Biochemistry, and Physiology by Uemura M (1997)
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10.1016/0304-4157(79)90012-1

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:58 p.m.)
Indexed	3 weeks, 1 day ago (Aug. 5, 2025, 8:24 a.m.)
Issued	26 years, 9 months ago (Nov. 24, 1998)
Published	26 years, 9 months ago (Nov. 24, 1998)
Published Online	26 years, 9 months ago (Nov. 24, 1998)
Published Print	26 years, 9 months ago (Nov. 24, 1998)

Funders 0

None

BibTeX

@article{Steponkus_1998, title={Mode of action of the COR15a gene on the freezing tolerance of Arabidopsis thaliana}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.24.14570}, DOI={10.1073/pnas.95.24.14570}, number={24}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Steponkus, Peter L. and Uemura, Matsuo and Joseph, Raymond A. and Gilmour, Sarah J. and Thomashow, Michael F.}, year={1998}, month=nov, pages={14570–14575} }

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