DOI: 10.1073/pnas.95.24.14066. DNA end-joining catalyzed by human cell-free extracts

DNA end-joining catalyzed by human cell-free extracts

10.1073/pnas.95.24.14066

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Mammalian cells defective in DNA end-joining are highly sensitive to ionizing radiation and are immunodeficient because of a failure to complete V(D)J recombination. By using cell-free extracts prepared from human lymphoblastoid cell lines, an in vitro system for end-joining has been developed. Intermolecular ligation was found to be accurate and to depend on DNA ligase IV/Xrcc4 and requires Ku70, Ku86, and DNA-PK cs , the three subunits of the DNA-activated protein kinase DNA-PK. Because these activities are involved in the cellular resistance to x-irradiation and V(D)J recombination, the development of this in vitro system provides an important advance in the study of the mechanism of DNA end-joining in human cells.

Bibliography

Baumann, P., & West, S. C. (1998). DNA end-joining catalyzed by human cell-free extracts. Proceedings of the National Academy of Sciences, 95(24), 14066â14070.

Authors 2

Peter Baumann (first)
Stephen C. West (additional)

References 48 Referenced 231

10.1016/0921-8777(95)00018-F
10.1016/0092-8674(95)90135-3
10.1016/S0960-9822(06)00258-2
10.1038/41358
10.1128/MCB.16.4.1519
10.1128/MCB.17.3.1264
10.1016/S0021-9258(17)33960-1
10.1016/0092-8674(95)90360-7
10.1016/0092-8674(95)90482-4
10.1016/S0021-9258(18)67534-9
10.1016/0092-8674(93)90057-W
10.1093/emboj/16.16.5098
10.1073/pnas.95.2.525
10.1074/jbc.271.44.27765
10.1074/jbc.271.14.7910
10.1128/MCB.16.8.4189
10.1093/genetics/142.1.91
10.1093/nar/24.23.4639
10.1002/j.1460-2075.1996.tb00890.x
10.1093/emboj/17.6.1819
10.1146/annurev.bi.61.070192.001343
10.1074/jbc.271.39.24257
10.1002/bies.950191009
10.1093/emboj/17.2.609
D Pang, S Yoo, W S Dynan, M Jung, A Dritschilo Cancer Res 57, 1412–1415 (1997). / Cancer Res by Pang D (1997)
10.1016/0165-1110(90)90028-A
10.1126/science.8469973
10.1038/41351
10.1006/bbrc.1995.2561
10.1093/nar/20.16.4145
10.1016/0921-8777(96)00028-6
10.1093/nar/24.24.4946
10.1021/bi962281m
10.1093/nar/22.13.2490
N D Lakin, P Weber, T Stankovic, S T Rottinghaus, A M Taylor, S P Jackson Oncogene 13, 2707–2716 (1996). / Oncogene by Lakin N D (1996)
10.1016/S0021-9258(19)38388-7
10.1093/nar/24.22.4387
10.1007/978-3-642-50140-1_7
10.1073/pnas.77.7.3855
10.1126/science.8073286
10.1074/jbc.272.9.5765
10.1073/pnas.94.9.4267
10.1074/jbc.271.24.14405
10.1074/jbc.273.3.1794
10.1101/gad.11.15.1912
10.1093/emboj/16.15.4788
10.1093/emboj/17.14.4188
10.1038/41365

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:32 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:57 p.m.)
Indexed	4 days, 18 hours ago (Aug. 27, 2025, 11:56 a.m.)
Issued	26 years, 9 months ago (Nov. 24, 1998)
Published	26 years, 9 months ago (Nov. 24, 1998)
Published Online	26 years, 9 months ago (Nov. 24, 1998)
Published Print	26 years, 9 months ago (Nov. 24, 1998)

Funders 0

None

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