The pir gene of Erwinia chrysanthemi EC16 regulates hyperinduction of pectate lyase virulence genes in response to plant signals
10.1073/pnas.95.24.14034
Crossref journal-article
Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences (341)
Abstract

The plant pathogenic bacterium Erwinia chrysanthemi secretes pectate lyase proteins that are important virulence factors attacking the cell walls of plant hosts. Bacterial production of these enzymes is induced by the substrate polypectate-Na (NaPP) and further stimulated by the presence of plant extracts. The bacterial regulator responsible for induction by plant extracts was identified and purified by using a DNA-binding assay with the promoter region of pelE that encodes a major pectate lyase. A novel bacterial protein, called Pir, was isolated that produced a specific gel shift of the pelE promoter DNA, and the corresponding pir gene was cloned and sequenced. The Pir protein contains 272 amino acids with a molecular mass of 30 kDa and appears to function as a dimer. A homology search indicates that Pir belongs to the IclR family of transcriptional regulators. Pir bound to a 35-bp DNA sequence in the promoter region of pelE . This site overlaps that of a previously described negative regulator, KdgR. Gel shift experiments showed that the binding of either Pir or KdgR interfered with binding of the other protein.

Bibliography

Nomura, K., Nasser, W., Kawagishi, H., & Tsuyumu, S. (1998). The pir gene of Erwinia chrysanthemi EC16 regulates hyperinduction of pectate lyase virulence genes in response to plant signals. Proceedings of the National Academy of Sciences, 95(24), 14034–14039.

Authors 4
  1. Kinya Nomura (first)
  2. William Nasser (additional)
  3. Hirokazu Kawagishi (additional)
  4. Shinji Tsuyumu (additional)
References 24 Referenced 44
  1. M C M Perombelon, A Kelman Annu Rev Phytopathol 18, 361–387 (1980). (10.1146/annurev.py.18.090180.002045) / Annu Rev Phytopathol by Perombelon M C M (1980)
  2. A Collmer, N T Keen Annu Rev Phytopathol 24, 383–409 (1986). (10.1146/annurev.py.24.090186.002123) / Annu Rev Phytopathol by Collmer A (1986)
  3. W Nasser, S Reverchon, J Robert-Baudouy Mol Microbiol 6, 257–265 (1992). (10.1111/j.1365-2958.1992.tb02007.x) / Mol Microbiol by Nasser W (1992)
  4. W Nasser, S Reverchon, G Condemine, J Robert-Baudouy J Mol Biol 236, 427–440 (1994). (10.1006/jmbi.1994.1155) / J Mol Biol by Nasser W (1994)
  5. N Hogouvieux-Cotte-Pattat, S Reverchon, G Condemine, J Robert-Baudouy J Gen Microbiol 132, 2099–2106 (1986). / J Gen Microbiol by Hogouvieux-Cotte-Pattat N (1986)
  6. N Hogouvieux-Cotte-Pattat, H Dominguez, J Robert-Baudouy J Bacteriol 174, 7807–7818 (1992). (10.1128/jb.174.23.7807-7818.1992) / J Bacteriol by Hogouvieux-Cotte-Pattat N (1992)
  7. C Sauvage, D Expert Mol Plant–Microbe Interact 7, 71–77 (1994). (10.1094/MPMI-7-0071) / Mol Plant–Microbe Interact by Sauvage C (1994)
  8. C Bourson, S Favey, S Reverchon, J Robert-Baudouy J Gen Microbiol 139, 1–9 (1993). (10.1099/00221287-139-1-1) / J Gen Microbiol by Bourson C (1993)
  9. N Hogouvieux-Cotte-Pattat, G Condemine, W Nasser, S Reverchon Annu Rev Microbiol 50, 213–257 (1996). (10.1146/annurev.micro.50.1.213) / Annu Rev Microbiol by Hogouvieux-Cotte-Pattat N (1996)
  10. Y Liu, H Murata, A Chatterjee, A K Chatterjee Mol Plant–Microbe Interact 6, 299–308 (1993). (10.1094/MPMI-6-299) / Mol Plant–Microbe Interact by Liu Y (1993)
  11. H Murata, A Chatterjee, Y Liu, A K Chatterjee Appl Environ Microbiol 60, 3150–3159 (1994). (10.1128/aem.60.9.3150-3159.1994) / Appl Environ Microbiol by Murata H (1994)
  12. A Chatterjee, Y Cui, Y Liu, C K Dumenyo, A K Chatterjee Appl Environ Microbiol 61, 1959–1967 (1995). (10.1128/aem.61.5.1959-1967.1995) / Appl Environ Microbiol by Chatterjee A (1995)
  13. F M Ausbel, R Brent, R E Kingston, D D Moore, J G Seidman, J A Smith, K Struhi Current Protocols in Molecular Biology (Wiley-Interscience, New York, 1987). / Current Protocols in Molecular Biology by Ausbel F M (1987)
  14. D J Galas, A Schmitz Nucleic Acids Res 5, 3157–3170 (1981). (10.1093/nar/5.9.3157) / Nucleic Acids Res by Galas D J (1981)
  15. J Sambrook, F Fritsch, T Maniatis Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Lab. Press, Plainview, NY, 1989). / Molecular Cloning: A Laboratory Manual by Sambrook J (1989)
  16. R A Jefferson, T A Kavanagh, M W Bevan EMBO J 6, 3901–3907 (1987). (10.1002/j.1460-2075.1987.tb02730.x) / EMBO J by Jefferson R A (1987)
  17. H Shen, S E Gold, S J Tamaki, N T Keen Gene 122, 27–34 (1992). (10.1016/0378-1119(92)90028-N) / Gene by Shen H (1992)
  18. G F Barry Gene 71, 75–84 (1988). (10.1016/0378-1119(88)90079-0) / Gene by Barry G F (1988)
  19. F R Blattner, G Plunkett, C A Bloch, N T Perna, V Burland, M Riley, J Collado-Vides, J D Glasner, C K Rode, G F Mayhew, et al. Science 277, 1453–1474 (1997). (10.1126/science.277.5331.1453) / Science by Blattner F R (1997)
  20. H J Sofia, V Burland, D L Daniels, G Plunkett, F R Blattner Nucleic Acids Res 22, 2576–2586 (1994). (10.1093/nar/22.13.2576) / Nucleic Acids Res by Sofia H J (1994)
  21. S Reverchon, W Nasser, J Robert-Baudouy Mol Microbiol 5, 2203–2216 (1991). (10.1111/j.1365-2958.1991.tb02150.x) / Mol Microbiol by Reverchon S (1991)
  22. H Bading Nucleic Acids Res 16, 5241–5248 (1988). (10.1093/nar/16.12.5241) / Nucleic Acids Res by Bading H (1988)
  23. S Gold, S Nishio, S Tsuyumu, N T Keen Mol Plant–Microbe Interact 2, 170–178 (1992). (10.1094/MPMI-5-170) / Mol Plant–Microbe Interact by Gold S (1992)
  24. J H Payne, C Schoedel, N T Keen, A Collmer Appl Environ Microbiol 53, 2315–2320 (1987). (10.1128/aem.53.10.2315-2320.1987) / Appl Environ Microbiol by Payne J H (1987)
Dates
Type When
Created 23 years, 1 month ago (July 26, 2002, 10:35 a.m.)
Deposited 3 years, 2 months ago (June 6, 2022, 11 p.m.)
Indexed 1 year, 9 months ago (Nov. 20, 2023, 6:55 a.m.)
Issued 26 years, 9 months ago (Nov. 24, 1998)
Published 26 years, 9 months ago (Nov. 24, 1998)
Published Online 26 years, 9 months ago (Nov. 24, 1998)
Published Print 26 years, 9 months ago (Nov. 24, 1998)
Funders 0

None

@article{Nomura_1998, title={The pir gene of Erwinia chrysanthemi EC16 regulates hyperinduction of pectate lyase virulence genes in response to plant signals}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.24.14034}, DOI={10.1073/pnas.95.24.14034}, number={24}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Nomura, Kinya and Nasser, William and Kawagishi, Hirokazu and Tsuyumu, Shinji}, year={1998}, month=nov, pages={14034–14039} }