DOI: 10.1073/pnas.95.23.13726. Derivation of pluripotent stem cells from cultured human primordial germ cells

Derivation of pluripotent stem cells from cultured human primordial germ cells

10.1073/pnas.95.23.13726

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Human pluripotent stem cells would be invaluable forin vitrostudies of aspects of human embryogenesis. With the goal of establishing pluripotent stem cell lines, gonadal ridges and mesenteries containing primordial germ cells (PGCs, 5–9 weeks postfertilization) were cultured on mouse STO fibroblast feeder layers in the presence of human recombinant leukemia inhibitory factor, human recombinant basic fibroblast growth factor, and forskolin. Initially, single PGCs in culture were visualized by alkaline phosphatase activity staining. Over a period of 7–21 days, PGCs gave rise to large multicellular colonies resembling those of mouse pluripotent stem cells termed embryonic stem and embryonic germ (EG) cells. Throughout the culture period most cells within the colonies continued to be alkaline phosphatase-positive and tested positive against a panel of five immunological markers (SSEA-1, SSEA-3, SSEA-4, TRA-1–60, and TRA-1–81) that have been used routinely to characterize embryonic stem and EG cells. The cultured cells have been continuously passaged and found to be karyotypically normal and stable. Both XX and XY cell cultures have been obtained. Immunohistochemical analysis of embryoid bodies collected from these cultures revealed a wide variety of differentiated cell types, including derivatives of all three embryonic germ layers. Based on their origin and demonstrated properties, these human PGC-derived cultures meet the criteria for pluripotent stem cells and most closely resemble EG cells.

Bibliography

Shamblott, M. J., Axelman, J., Wang, S., Bugg, E. M., Littlefield, J. W., Donovan, P. J., Blumenthal, P. D., Huggins, G. R., & Gearhart, J. D. (1998). Derivation of pluripotent stem cells from cultured human primordial germ cells. Proceedings of the National Academy of Sciences, 95(23), 13726â13731.

Authors 9

Michael J. Shamblott (first)
Joyce Axelman (additional)
Shunping Wang (additional)
Elizabeth M. Bugg (additional)
John W. Littlefield (additional)
Peter J. Donovan (additional)
Paul D. Blumenthal (additional)
George R. Huggins (additional)
John D. Gearhart (additional)

References 40 Referenced 1,064

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10.1128/mcb.13.1.473-486.1993
M Klug, M Soonpaa, L Field Am J Physiol 269, H1913–H1921 (1995). / Am J Physiol by Klug M (1995)
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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:40 a.m.)
Deposited	2 years, 4 months ago (April 22, 2023, 8:23 a.m.)
Indexed	3 days, 20 hours ago (Aug. 28, 2025, 8:02 a.m.)
Issued	26 years, 9 months ago (Nov. 10, 1998)
Published	26 years, 9 months ago (Nov. 10, 1998)
Published Online	26 years, 9 months ago (Nov. 10, 1998)
Published Print	26 years, 9 months ago (Nov. 10, 1998)

Funders 0

None

BibTeX

@article{Shamblott_1998, title={Derivation of pluripotent stem cells from cultured human primordial germ cells}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.23.13726}, DOI={10.1073/pnas.95.23.13726}, number={23}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Shamblott, Michael J. and Axelman, Joyce and Wang, Shunping and Bugg, Elizabeth M. and Littlefield, John W. and Donovan, Peter J. and Blumenthal, Paul D. and Huggins, George R. and Gearhart, John D.}, year={1998}, month=nov, pages={13726–13731} }

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