DOI: 10.1073/pnas.95.22.12961. Direct molecular level measurements of the electrostatic properties of a protein surface

Direct molecular level measurements of the electrostatic properties of a protein surface

10.1073/pnas.95.22.12961

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

In this work, we used direct measurements with the surface force apparatus to determine the pH-dependent electrostatic charge density of a single binding face of streptavidin. Mean field calculations have been used with considerable success to model electrostatic potential fields near protein surfaces, but these models and their inherent assumptions have not been tested directly at the molecular level. Using the force apparatus and immobilized, oriented monolayers of streptavidin, we measured a pI of 5–5.5 for the biotin-binding face of the protein. This differs from the pI of 6.3 for the soluble protein and confirms that we probed the local electrostatic features of the macromolecule. With finite difference solutions of the linearized Poisson–Boltzmann equation, we then calculated the pH-dependent charge densities adjacent to the same face of the protein. These calculated values agreed quantitatively with those obtained by direct force measurements. Although our study focuses on the pH-dependence of surface electrostatics, this direct approach to probing the electrostatic features of proteins is applicable to investigations of any perturbations that alter the charge distribution of the surfaces of immobilized molecules.

Bibliography

Sivasankar, S., Subramaniam, S., & Leckband, D. (1998). Direct molecular level measurements of the electrostatic properties of a protein surface. Proceedings of the National Academy of Sciences, 95(22), 12961â12966.

Authors 3

S. Sivasankar (first)
S. Subramaniam (additional)
D. Leckband (additional)

References 40 Referenced 85

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:42 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:05 p.m.)
Indexed	3 months, 2 weeks ago (May 15, 2025, 12:25 p.m.)
Issued	26 years, 10 months ago (Oct. 27, 1998)
Published	26 years, 10 months ago (Oct. 27, 1998)
Published Online	26 years, 10 months ago (Oct. 27, 1998)
Published Print	26 years, 10 months ago (Oct. 27, 1998)

Funders 0

None

BibTeX

@article{Sivasankar_1998, title={Direct molecular level measurements of the electrostatic properties of a protein surface}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.22.12961}, DOI={10.1073/pnas.95.22.12961}, number={22}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Sivasankar, S. and Subramaniam, S. and Leckband, D.}, year={1998}, month=oct, pages={12961–12966} }

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