DOI: 10.1073/pnas.95.17.9855. Acetylation and modulation of erythroid Krüppel-like factor (EKLF) activity by interaction with histone acetyltransferases

Acetylation and modulation of erythroid Krüppel-like factor (EKLF) activity by interaction with histone acetyltransferases

10.1073/pnas.95.17.9855

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Erythroid Krüppel-like factor (EKLF) is a red cell-specific transcriptional activator that is crucial for consolidating the switch to high levels of adult β-globin expression during erythroid ontogeny. EKLF is required for integrity of the chromatin structure at the β-like globin locus, and it interacts with a positive-acting factor in vivo . We find that EKLF is an acetylated transcription factor, and that it interacts in vivo with CBP, p300, and P/CAF. However, its interactions with these histone acetyltransferases are not equivalent, as CBP and p300, but not P/CAF, utilize EKLF as a substrate for in vitro acetylation within its trans-activation region. The functional effects of these interactions are that CBP and p300, but not P/CAF, enhance EKLF’s transcriptional activation of the β-globin promoter in erythroid cells. These results establish EKLF as a tissue-specific transcription factor that undergoes post-translational acetylation and suggest a mechanism by which EKLF is able to alter chromatin structure and induce β-globin expression within the β-like globin cluster.

Bibliography

Zhang, W., & Bieker, J. J. (1998). Acetylation and modulation of erythroid KrÃ¼ppel-like factor (EKLF) activity by interaction with histone acetyltransferases. Proceedings of the National Academy of Sciences, 95(17), 9855â9860.

Authors 2

Wenjun Zhang (first)
James J. Bieker (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:32 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 5:12 p.m.)
Indexed	3 weeks, 2 days ago (Aug. 5, 2025, 8:31 a.m.)
Issued	27 years ago (Aug. 18, 1998)
Published	27 years ago (Aug. 18, 1998)
Published Online	27 years ago (Aug. 18, 1998)
Published Print	27 years ago (Aug. 18, 1998)

Funders 0

None

BibTeX

@article{Zhang_1998, title={Acetylation and modulation of erythroid Krüppel-like factor (EKLF) activity by interaction with histone acetyltransferases}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.17.9855}, DOI={10.1073/pnas.95.17.9855}, number={17}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Zhang, Wenjun and Bieker, James J.}, year={1998}, month=aug, pages={9855–9860} }

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