DOI: 10.1073/pnas.95.17.10106. Epstein–Barr virus-transforming protein latent infection membrane protein 1 activates transcription factor NF-κB through a pathway that includes the NF-κB-inducing kinase and the IκB kinases IKKα and IKKβ

Epstein–Barr virus-transforming protein latent infection membrane protein 1 activates transcription factor NF-κB through a pathway that includes the NF-κB-inducing kinase and the IκB kinases IKKα and IKKβ

10.1073/pnas.95.17.10106

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The Epstein–Barr virus oncoprotein latent infection membrane protein 1 (LMP1) is a constitutively aggregated pseudo-tumor necrosis factor receptor (TNFR) that activates transcription factor NF-κB through two sites in its C-terminal cytoplasmic domain. One site is similar to activated TNFRII in associating with TNFR-associated factors TRAF1 and TRAF2, and the second site is similar to TNFRI in associating with the TNFRI death domain interacting protein TRADD. TNFRI has been recently shown to activate NF-κB through association with TRADD, RIP, and TRAF2; activation of the NF-κB-inducing kinase (NIK); activation of the IκBα kinases (IKKα and IKKβ); and phosphorylation of IκBα. IκBα phosphorylation on Ser-32 and Ser-36 is followed by its degradation and NF-κB activation. In this report, we show that NF-κB activation by LMP1 or by each of its effector sites is mediated by a pathway that includes NIK, IKKα, and IKKβ. Dominant negative mutants of NIK, IKKα, or IKKβ substantially inhibited NF-κB activation by LMP1 or by each of its effector sites.

Bibliography

Sylla, B. S., Hung, S. C., Davidson, D. M., Hatzivassiliou, E., Malinin, N. L., Wallach, D., Gilmore, T. D., Kieff, E., & Mosialos, G. (1998). EpsteinâBarr virus-transforming protein latent infection membrane protein 1 activates transcription factor NF-ÎºB through a pathway that includes the NF-ÎºB-inducing kinase and the IÎºB kinases IKKÎ± and IKKÎ². Proceedings of the National Academy of Sciences, 95(17), 10106â10111.

Authors 9

Bakary S. Sylla (first)
Siu Chun Hung (additional)
David M. Davidson (additional)
Eudoxia Hatzivassiliou (additional)
Nikolai L. Malinin (additional)
David Wallach (additional)
Thomas D. Gilmore (additional)
Elliott Kieff (additional)
George Mosialos (additional)

References 41 Referenced 129

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:42 a.m.)
Deposited	2 years, 4 months ago (April 22, 2023, 8:29 a.m.)
Indexed	3 months ago (May 26, 2025, 1:23 a.m.)
Issued	27 years ago (Aug. 18, 1998)
Published	27 years ago (Aug. 18, 1998)
Published Online	27 years ago (Aug. 18, 1998)
Published Print	27 years ago (Aug. 18, 1998)

Funders 0

None

BibTeX

@article{Sylla_1998, title={Epstein–Barr virus-transforming protein latent infection membrane protein 1 activates transcription factor NF-κB through a pathway that includes the NF-κB-inducing kinase and the IκB kinases IKKα and IKKβ}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.17.10106}, DOI={10.1073/pnas.95.17.10106}, number={17}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Sylla, Bakary S. and Hung, Siu Chun and Davidson, David M. and Hatzivassiliou, Eudoxia and Malinin, Nikolai L. and Wallach, David and Gilmore, Thomas D. and Kieff, Elliott and Mosialos, George}, year={1998}, month=aug, pages={10106–10111} }

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