DOI: 10.1073/pnas.95.16.9337. Direct binding of follistatin to a complex of bone-morphogenetic protein and its receptor inhibits ventral and epidermal cell fates in early <i>Xenopus</i> embryo

Direct binding of follistatin to a complex of bone-morphogenetic protein and its receptor inhibits ventral and epidermal cell fates in early Xenopus embryo

10.1073/pnas.95.16.9337

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

In early development of Xenopus laevis, it is known that activities of polypeptide growth factors are negatively regulated by their binding proteins. In this study, follistatin, originally known as an activin-binding protein, was shown to inhibit all aspects of bone morphogenetic protein (BMP) activity in early Xenopus embryos. Furthermore, using a surface plasmon resonance biosensor, we demonstrated that follistatin can directly interact with multiple BMPs at significantly high affinities. Interestingly, follistatin was found to be noncompetitive with the BMP receptor for ligand binding and to form a trimeric complex with BMP and its receptor. The results suggest that follistatin acts as an organizer factor in early amphibian embryogenesis by inhibiting BMP activities by a different mechanism from that used by chordin and noggin.

Bibliography

Iemura, S., Yamamoto, T. S., Takagi, C., Uchiyama, H., Natsume, T., Shimasaki, S., Sugino, H., & Ueno, N. (1998). Direct binding of follistatin to a complex of bone-morphogenetic protein and its receptor inhibits ventral and epidermal cell fates in early Xenopus embryo. Proceedings of the National Academy of Sciences, 95(16), 9337â9342.

Authors 8

Shun-ichiro Iemura (first)
Takamasa S. Yamamoto (additional)
Chiyo Takagi (additional)
Hideho Uchiyama (additional)
Tohru Natsume (additional)
Shunichi Shimasaki (additional)
Hiromu Sugino (additional)
Naoto Ueno (additional)

References 38 Referenced 357

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:40 p.m.)
Indexed	1 month, 1 week ago (July 24, 2025, 7:23 a.m.)
Issued	27 years ago (Aug. 4, 1998)
Published	27 years ago (Aug. 4, 1998)
Published Online	27 years ago (Aug. 4, 1998)
Published Print	27 years ago (Aug. 4, 1998)

Funders 0

None

BibTeX

@article{Iemura_1998, title={Direct binding of follistatin to a complex of bone-morphogenetic protein and its receptor inhibits ventral and epidermal cell fates in early Xenopus embryo}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.16.9337}, DOI={10.1073/pnas.95.16.9337}, number={16}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Iemura, Shun-ichiro and Yamamoto, Takamasa S. and Takagi, Chiyo and Uchiyama, Hideho and Natsume, Tohru and Shimasaki, Shunichi and Sugino, Hiromu and Ueno, Naoto}, year={1998}, month=aug, pages={9337–9342} }

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