DOI: 10.1073/pnas.95.15.8733. Bloom’s and Werner’s syndrome genes suppress hyperrecombination in yeast <i>sgs1</i> mutant: Implication for genomic instability in human diseases

Bloom’s and Werner’s syndrome genes suppress hyperrecombination in yeast sgs1 mutant: Implication for genomic instability in human diseases

10.1073/pnas.95.15.8733

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Bloom’s syndrome (BS) and Werner’s syndrome (WS) are genetic disorders in which an increased rate of chromosomal aberration is detected. The genes responsible for these diseases, BLM and WRN , have been found to be homologs of Escherichia coli recQ and Saccharomyces cerevisiae SGS1 genes. Here we show that yeast Sgs1 helicase acts as a suppressor of illegitimate recombination through homologous recombination and that human BLM and WRN helicases can suppress the increased homologous and illegitimate recombinations in the S. cerevisiae sgs1 mutant. The results imply a role of BLM and WRN helicases to control genomic stability in human cells. Similar to Sgs1 helicase, BLM helicase suppressed the cell growth in the top3 sgs1 mutation background and restored the increased sensitivity of the sgs1 mutant to hydroxyurea, but the WRN helicase did not. We discussed differential roles of BLM and WRN helicases in human cells. BLM- and WRN-bearing yeasts provide new useful models to investigate human BS and WS diseases.

Bibliography

Yamagata, K., Kato, J., Shimamoto, A., Goto, M., Furuichi, Y., & Ikeda, H. (1998). Bloomâs and Wernerâs syndrome genes suppress hyperrecombination in yeast sgs1 mutant: Implication for genomic instability in human diseases. Proceedings of the National Academy of Sciences, 95(15), 8733â8738.

Authors 6

Kazutsune Yamagata (first)
Jun-ichi Kato (additional)
Akira Shimamoto (additional)
Makoto Goto (additional)
Yasuhiro Furuichi (additional)
Hideo Ikeda (additional)

References 30 Referenced 221

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:32 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:29 p.m.)
Indexed	3 weeks, 4 days ago (Aug. 5, 2025, 8:38 a.m.)
Issued	27 years, 1 month ago (July 21, 1998)
Published	27 years, 1 month ago (July 21, 1998)
Published Online	27 years, 1 month ago (July 21, 1998)
Published Print	27 years, 1 month ago (July 21, 1998)

Funders 0

None

BibTeX

@article{Yamagata_1998, title={Bloom’s and Werner’s syndrome genes suppress hyperrecombination in yeast sgs1 mutant: Implication for genomic instability in human diseases}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.15.8733}, DOI={10.1073/pnas.95.15.8733}, number={15}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Yamagata, Kazutsune and Kato, Jun-ichi and Shimamoto, Akira and Goto, Makoto and Furuichi, Yasuhiro and Ikeda, Hideo}, year={1998}, month=jul, pages={8733–8738} }

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