DOI: 10.1073/pnas.95.15.8590. The proton collecting function of the inner surface of cytochrome<i>c</i>oxidase from<i>Rhodobacter sphaeroides</i>

The proton collecting function of the inner surface of cytochromecoxidase fromRhodobacter sphaeroides

10.1073/pnas.95.15.8590

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The experiments presented in this study address the problem of how the cytoplasmic surface (proton-input side) of cytochromecoxidase interacts with protons in the bulk. For this purpose, the cytoplasmic surface of the enzyme was labeled with a fluorescein (Flu) molecule covalently bound to Cys223 of subunit III. Using the Flu as a proton-sensitive marker on the surface and φOH as a soluble excited-state proton emitter, the dynamics of the acid-base equilibration between the surface and the bulk was measured in the time-resolved domain. The results were analyzed by using a rigorous kinetic analysis that is based on numeric integration of coupled nonliner differential rate equations in which the rate constants are used as adjustable parameters. The analysis of 11 independent measurements, carried out under various initial conditions, indicated that the protonation of the Flu proceeds through multiple pathways involving diffusion-controlled reactions and proton exchange among surface groups. The surface of the protein carries an efficient system made of carboxylate and histidine moieties that are sufficiently close to each other as to form a proton-collecting antenna. It is the passage of protons among these sites that endows cytochromecoxidase with the capacity to pick up protons from the buffered cytoplasmic matrix within a time frame compatible with the physiological turnover of the enzyme.

Bibliography

Marantz, Y., Nachliel, E., Aagaard, A., Brzezinski, P., & Gutman, M. (1998). The proton collecting function of the inner surface of cytochromecoxidase fromRhodobacter sphaeroides. Proceedings of the National Academy of Sciences, 95(15), 8590â8595.

Authors 5

Yael Marantz (first)
Esther Nachliel (additional)
Anna Aagaard (additional)
Peter Brzezinski (additional)
Menachem Gutman (additional)

References 31 Referenced 72

10.1016/0022-2860(94)87019-5
10.1016/S0065-3233(08)60197-7
M Gutman, E Nachliel, Y Tsfadia Permeability and Stability of Lipid Bilayers, eds E A Disalvo, S A Simon (CRC, Boca Raton, FL), pp. 259–276 (1995). / Permeability and Stability of Lipid Bilayers by Gutman M (1995)
10.1073/pnas.93.20.10747
10.1021/bi9717542
10.1021/cr950051s
10.1038/376660a0
10.1126/science.7652554
10.1126/science.272.5265.1136
10.1007/BF00762854
10.1021/bi00091a046
10.1021/bi9629079
10.1073/pnas.94.17.9085
10.1021/bi971813b
Brzezinski P. & Ädelroth P. (1998) J. Bioenerg. Biomembr. in press.
M Gutman Methods Biochem Anal 30, 1–103 (1984). (10.1002/9780470110515.ch1) / Methods Biochem Anal by Gutman M (1984)
10.1016/0076-6879(86)27042-1
Marantz Y. Nachliel E. Aagaard A. Brzezinski P. & Gutman M. (1998) Biochim. Biophys. Acta. in press.
10.1016/0014-5793(95)00626-K
10.1021/bi00867a005
V Sacks M.Sc. thesis (Tel Aviv University, Ramat Aviv, Israel, 1997). / M.Sc. thesis by Sacks V (1997)
Sacks V. Marantz Y. Aagaard A. Checover S. Nachliel E. & Gutman M. (1998) Biochim. Biophys. Acta in press.
10.1021/bi00333a020
10.1016/0005-2728(90)90073-D
10.1146/annurev.physchem.48.1.329
10.1021/ja00216a044
10.1016/0005-2728(95)00074-S
10.1016/S0005-2728(89)80087-8
10.1073/pnas.93.22.12292
10.1021/bi960260m
10.1006/jmbi.1993.1626

Dates

Type	When
Created	23 years ago (July 26, 2002, 10:39 a.m.)
Deposited	1 year, 7 months ago (Jan. 4, 2024, 12:01 a.m.)
Indexed	11 months, 2 weeks ago (Sept. 9, 2024, 10:44 a.m.)
Issued	27 years, 1 month ago (July 21, 1998)
Published	27 years, 1 month ago (July 21, 1998)
Published Online	27 years, 1 month ago (July 21, 1998)
Published Print	27 years, 1 month ago (July 21, 1998)

Funders 0

None

BibTeX

@article{Marantz_1998, title={The proton collecting function of the inner surface of cytochromecoxidase fromRhodobacter sphaeroides}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.15.8590}, DOI={10.1073/pnas.95.15.8590}, number={15}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Marantz, Yael and Nachliel, Esther and Aagaard, Anna and Brzezinski, Peter and Gutman, Menachem}, year={1998}, month=jul, pages={8590–8595} }

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