DOI: 10.1073/pnas.95.15.8514. Activities of adenovirus virus-associated RNAs: Purification and characterization of RNA binding proteins

Activities of adenovirus virus-associated RNAs: Purification and characterization of RNA binding proteins

10.1073/pnas.95.15.8514

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Most human adenoviruses encode two virus-associated (VA) RNAs, VA RNA I and VA RNA II , that accumulate to high levels in the cytoplasm of infected cells. The function of VA RNA I in blocking the activation of the cellular kinase PKR is well known, but the role of VA RNA II is obscure. Herein we characterize and purify several human proteins that interact preferentially with VA RNA II in Northwestern blot assays. Two of these proteins were identified as RNA helicase A and NF90, a component of the heterodimeric nuclear factor of activated T cells (NFAT). They copurified with the smaller NFAT subunit, NF45, which did not bind VA RNA II , and with an unidentified protein, p97, which did bind VA RNA II . Both RNA helicase A and NF90 contain two copies of a double-stranded (ds) RNA binding motif and bind strongly to dsRNA. NF90 interacts with RNAs in the following order of affinity: dsRNA > VA RNA II > VA RNA I > single-stranded RNA. Furthermore, VA RNA II is more effective than VA RNA I as an inhibitor of RNA helicase activity. These data identify RNA helicase A and NF90 as cellular proteins with an affinity for dsRNA and other structured RNA molecules and suggest that their functions are subject to regulation by RNA ligands including VA RNA II .

Bibliography

Liao, H.-J., Kobayashi, R., & Mathews, M. B. (1998). Activities of adenovirus virus-associated RNAs: Purification and characterization of RNA binding proteins. Proceedings of the National Academy of Sciences, 95(15), 8514â8519.

Authors 3

Huey-Jane Liao (first)
Ryuji Kobayashi (additional)
Michael B. Mathews (additional)

References 45 Referenced 81

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:39 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:39 p.m.)
Indexed	1 month, 3 weeks ago (July 8, 2025, 1:45 a.m.)
Issued	27 years, 1 month ago (July 21, 1998)
Published	27 years, 1 month ago (July 21, 1998)
Published Online	27 years, 1 month ago (July 21, 1998)
Published Print	27 years, 1 month ago (July 21, 1998)

Funders 0

None

BibTeX

@article{Liao_1998, title={Activities of adenovirus virus-associated RNAs: Purification and characterization of RNA binding proteins}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.15.8514}, DOI={10.1073/pnas.95.15.8514}, number={15}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Liao, Huey-Jane and Kobayashi, Ryuji and Mathews, Michael B.}, year={1998}, month=jul, pages={8514–8519} }

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