DOI: 10.1073/pnas.95.14.8026. Inhibitor binding changes domain mobility in the iron–sulfur protein of the mitochondrial <i>bc</i> 1 complex from bovine heart

Inhibitor binding changes domain mobility in the iron–sulfur protein of the mitochondrial bc 1 complex from bovine heart

10.1073/pnas.95.14.8026

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We have analyzed crystal structures of cytochrome bc 1 complexes with electron transfer inhibitors bound to the ubiquinone binding pockets Q i and/or Q o in the cytochrome b subunit. The presence or absence of the Q i inhibitor antimycin A did not affect the binding of the Q o inhibitors. Different subtypes of Q o inhibitors had dramatically different effects on the mobility of the extramembrane domain of the iron–sulfur protein (ISP): Binding of 5-undecyl-6-hydroxy-4,7-dioxobenzothiazol and stigmatellin (subtype Q o –II and Q o –III, respectively) led to a fixation of the ISP domain on the surface of cytochrome b , whereas binding of myxothiazol and methoxyacrylate-stilbene (subtype Q o –I) favored release of this domain. The native structure has an empty Q o pocket and is intermediate between these extremes. On the basis of these observations we propose a model of quinone oxidation in the bc 1 complex, which incorporates fixed and loose states of the ISP as features important for electron transfer and, possibly, also proton transport.

Bibliography

Kim, H., Xia, D., Yu, C.-A., Xia, J.-Z., Kachurin, A. M., Zhang, L., Yu, L., & Deisenhofer, J. (1998). Inhibitor binding changes domain mobility in the ironâsulfur protein of the mitochondrial bc 1 complex from bovine heart. Proceedings of the National Academy of Sciences, 95(14), 8026â8033.

Authors 8

Hoeon Kim (first)
Di Xia (additional)
Chang-An Yu (additional)
Jia-Zhi Xia (additional)
Anatoly M. Kachurin (additional)
Li Zhang (additional)
Linda Yu (additional)
Johann Deisenhofer (additional)

References 34 Referenced 231

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:39 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:46 p.m.)
Indexed	4 weeks ago (Aug. 6, 2025, 9:42 a.m.)
Issued	27 years, 1 month ago (July 7, 1998)
Published	27 years, 1 month ago (July 7, 1998)
Published Online	27 years, 1 month ago (July 7, 1998)
Published Print	27 years, 1 month ago (July 7, 1998)

Funders 0

None

BibTeX

@article{Kim_1998, title={Inhibitor binding changes domain mobility in the iron–sulfur protein of the mitochondrial bc 1 complex from bovine heart}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.14.8026}, DOI={10.1073/pnas.95.14.8026}, number={14}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kim, Hoeon and Xia, Di and Yu, Chang-An and Xia, Jia-Zhi and Kachurin, Anatoly M. and Zhang, Li and Yu, Linda and Deisenhofer, Johann}, year={1998}, month=jul, pages={8026–8033} }

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