DOI: 10.1073/pnas.95.14.7969. The SbcCD nuclease of <i>Escherichia coli</i> is a structural maintenance of chromosomes (SMC) family protein that cleaves hairpin DNA

The SbcCD nuclease of Escherichia coli is a structural maintenance of chromosomes (SMC) family protein that cleaves hairpin DNA

10.1073/pnas.95.14.7969

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Hairpin structures can inhibit DNA replication and are intermediates in certain recombination reactions. We have shown that the purified SbcCD protein of Escherichia coli cleaves a DNA hairpin. This cleavage does not require the presence of a free (3′ or 5′) DNA end and generates products with 3′-hydroxyl and 5′-phosphate termini. Electron microscopy of SbcCD has revealed the “head-rod-tail” structure predicted for the SMC (structural maintenance of chromosomes) family of proteins, of which SbcC is a member. This work provides evidence consistent with the proposal that SbcCD cleaves hairpin structures that halt the progress of the replication fork, allowing homologous recombination to restore DNA replication.

Bibliography

Connelly, J. C., Kirkham, L. A., & Leach, D. R. F. (1998). The SbcCD nuclease of Escherichia coli is a structural maintenance of chromosomes (SMC) family protein that cleaves hairpin DNA. Proceedings of the National Academy of Sciences, 95(14), 7969â7974.

Authors 3

John C. Connelly (first)
Lucy A. Kirkham (additional)
David R. F. Leach (additional)

References 73 Referenced 184

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:39 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:47 p.m.)
Indexed	1 month ago (Aug. 2, 2025, 12:53 a.m.)
Issued	27 years, 1 month ago (July 7, 1998)
Published	27 years, 1 month ago (July 7, 1998)
Published Online	27 years, 1 month ago (July 7, 1998)
Published Print	27 years, 1 month ago (July 7, 1998)

Funders 0

None

BibTeX

@article{Connelly_1998, title={The SbcCD nuclease of Escherichia coli is a structural maintenance of chromosomes (SMC) family protein that cleaves hairpin DNA}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.14.7969}, DOI={10.1073/pnas.95.14.7969}, number={14}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Connelly, John C. and Kirkham, Lucy A. and Leach, David R. F.}, year={1998}, month=jul, pages={7969–7974} }

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