Abstract
Cognate cDNAs are described for 2 of the 10 thyroid hormone receptor-associated proteins (TRAPs) that are immunopurified with thyroid hormone receptor α (TRα) from ligand-treated HeLa (α-2) cells. Both TRAP220 and TRAP100 contain LXXLL domains found in other nuclear receptor-interacting proteins and both appear to reside in a single complex with other TRAPs (in the absence of TR). However, only TRAP220 shows a direct ligand-dependent interaction with TRα, and these interactions are mediated through the C terminus of TRα and (at least in part) the LXXLL domains of TRAP220. TRAP220 also interacts with other nuclear receptors [vitamin D receptor, retinoic acid receptor α, retinoid X receptor α, peroxisome proliferation-activated receptor (PPAR) α, PPARγ and, to a lesser extent, estrogen receptor] in a ligand-dependent manner, whereas TRAP100 shows only marginal interactions with estrogen receptor, retinoid X receptor α, PPARα, and PPARγ. Consistent with these results, TRAP220 moderately stimulates human TRα-mediated transcription in transfected cells, whereas a fragment containing the LXXLL motifs acts as a dominant negative inhibitor of nuclear receptor-mediated transcription both in transfected cells (TRα) and in cell free transcription systems (TRα and vitamin D receptor). These studies indicate that TRAP220 plays a major role in anchoring other TRAPs to TRα during the function of the TRα–TRAP complex and, further, that TRAP220 (possibly along with other TRAPs) may be a global coactivator for the nuclear receptor superfamily.
Bibliography
Yuan, C.-X., Ito, M., Fondell, J. D., Fu, Z.-Y., & Roeder, R. G. (1998). The TRAP220 component of a thyroid hormone receptor- associated protein (TRAP) coactivator complex interacts directly with nuclear receptors in a ligand-dependent fashion. Proceedings of the National Academy of Sciences, 95(14), 7939â7944.
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Dates
| Type | When |
|---|---|
| Created | 23 years, 1 month ago (July 26, 2002, 10:39 a.m.) |
| Deposited | 3 years, 2 months ago (June 6, 2022, 9:31 p.m.) |
| Indexed | 19 minutes ago (Aug. 29, 2025, 2:06 a.m.) |
| Issued | 27 years, 1 month ago (July 7, 1998) |
| Published | 27 years, 1 month ago (July 7, 1998) |
| Published Online | 27 years, 1 month ago (July 7, 1998) |
| Published Print | 27 years, 1 month ago (July 7, 1998) |
@article{Yuan_1998, title={The TRAP220 component of a thyroid hormone receptor- associated protein (TRAP) coactivator complex interacts directly with nuclear receptors in a ligand-dependent fashion}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.14.7939}, DOI={10.1073/pnas.95.14.7939}, number={14}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Yuan, Chao-Xing and Ito, Mitsuhiro and Fondell, Joseph D. and Fu, Zheng-Yuan and Roeder, Robert G.}, year={1998}, month=jul, pages={7939–7944} }