DOI: 10.1073/pnas.95.13.7299. Supramolecular organization of immature and mature murine leukemia virus revealed by electron cryo-microscopy: Implications for retroviral assembly mechanisms

Supramolecular organization of immature and mature murine leukemia virus revealed by electron cryo-microscopy: Implications for retroviral assembly mechanisms

10.1073/pnas.95.13.7299

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We have used electron cryo-microscopy and image analysis to examine the native structure of immature, protease-deficient (PR − ) and mature, wild-type (WT) Moloney murine leukemia virus (MuLV). Maturational cleavage of the Gag polyprotein by the viral protease is associated with striking morphological changes. The PR − MuLV particles exhibit a rounded central core, which has a characteristic track-like shell on its surface, whereas the WT MuLV cores display a polygonal surface with loss of the track-like feature. The pleomorphic shape and inability to refine unique orientation angles suggest that neither the PR − nor the WT MuLV adheres to strict icosahedral symmetry. Nevertheless, the PR − MuLV particles do exhibit paracrystalline order with a spacing between Gag molecules of ≈45 Å and a length of ≈200 Å. Because of the pleomorphic shape and paracrystalline packing of the Gag–RNA complexes, we raise the possibility that assembly of MuLV is driven by protein–RNA, as well as protein–protein, interactions. The maturation process involves a dramatic reorganization of the packing arrangements within the ribonucleoprotein core with disordering and loosening of the individual protein components.

Bibliography

Yeager, M., Wilson-Kubalek, E. M., Weiner, S. G., Brown, P. O., & Rein, A. (1998). Supramolecular organization of immature and mature murine leukemia virus revealed by electron cryo-microscopy: Implications for retroviral assembly mechanisms. Proceedings of the National Academy of Sciences, 95(13), 7299â7304.

Authors 5

Mark Yeager (first)
Elizabeth M. Wilson-Kubalek (additional)
Scott G. Weiner (additional)
Patrick O. Brown (additional)
Alan Rein (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 10:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:01 p.m.)
Indexed	1 year ago (Aug. 12, 2024, 7:17 a.m.)
Issued	27 years, 1 month ago (June 23, 1998)
Published	27 years, 1 month ago (June 23, 1998)
Published Online	27 years, 1 month ago (June 23, 1998)
Published Print	27 years, 1 month ago (June 23, 1998)

Funders 0

None

BibTeX

@article{Yeager_1998, title={Supramolecular organization of immature and mature murine leukemia virus revealed by electron cryo-microscopy: Implications for retroviral assembly mechanisms}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.13.7299}, DOI={10.1073/pnas.95.13.7299}, number={13}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Yeager, Mark and Wilson-Kubalek, Elizabeth M. and Weiner, Scott G. and Brown, Patrick O. and Rein, Alan}, year={1998}, month=jun, pages={7299–7304} }

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