DOI: 10.1073/pnas.95.12.7174. A release mechanism for stored ATP in ocular ciliary epithelial cells [Penn]

A release mechanism for stored ATP in ocular ciliary epithelial cells

10.1073/pnas.95.12.7174

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Purines can modify ciliary epithelial secretion of aqueous humor into the eye. The source of the purinergic agonists acting in the ciliary epithelium, as in many epithelial tissues, is unknown. We found that the fluorescent ATP marker quinacrine stained rabbit and bovine ciliary epithelia but not the nerve fibers in the ciliary bodies. Cultured bovine pigmented and nonpigmented ciliary epithelial cells also stained intensely when incubated with quinacrine. Hypotonic stimulation of cultured epithelial cells increased the extracellular ATP concentration by 3-fold; this measurement underestimates actual release as the cells also displayed ecto-ATPase activity. The hypotonically triggered increase in ATP was inhibited by the Cl − -channel blocker 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) in both cell types. In contrast, the P-glycoprotein inhibitors tamoxifen and verapamil and the cystic fibrosis transmembrane conductance regulator (CFTR) blockers glybenclamide and diphenylamine-2-carboxylate did not affect ATP release from either cell type. This pharmacological profile suggests that ATP release is not restricted to P-glycoprotein or the cystic fibrosis transmembrane conductance regulator, but can proceed through a route sensitive to NPPB. ATP release also was triggered by ionomycin through a different NPPB-insensitive mechanism, inhibitable by the calcium/calmodulin-activated kinase II inhibitor KN-62. Thus, both layers of the ciliary epithelium store and release ATP, and purines likely modulate aqueous humor flow by paracrine and/or autocrine mechanisms within the two cell layers of this epithelium.

Bibliography

Mitchell, C. H., CarrÃ©, D. A., McGlinn, A. M., Stone, R. A., & Civan, M. M. (1998). A release mechanism for stored ATP in ocular ciliary epithelial cells. Proceedings of the National Academy of Sciences, 95(12), 7174â7178.

Authors 5

Claire H. Mitchell (first)
David A. Carré (additional)
Alice M. McGlinn (additional)
Richard A. Stone (additional)
Mortimer M. Civan (additional)

References 35 Referenced 189

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C E Crosson J Pharmacol Exp Ther 273, 320–326 (1995). / J Pharmacol Exp Ther by Crosson C E (1995)
C E Crosson, T Gray Invest Ophthalmol Visual Sci 37, 1833–1839 (1996). / Invest Ophthalmol Visual Sci by Crosson C E (1996)
Mitchell C. H. Peterson-Yantorno K. Coca-Prados M. & Civan M. M. (1997) Invest. Ophthalmol. Visual Sci. 38 Suppl. S1042 (abstr.).
Petrovich M. Gray T. & Crosson C. E. (1997) Invest. Ophthalmol. Visual Sci. 38 Suppl. S820 (abstr.).
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G E Knight, C H Hoyle, G Burnstock Comp Biochem Physiol 102, 305–314 (1992). / Comp Biochem Physiol by Knight G E (1992)
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C A McCannel, P D Scanlon, S Thibodeau, R F Brubaker Invest Ophthalmol Visual Sci 33, 160–164 (1992). / Invest Ophthalmol Visual Sci by McCannel C A (1992)
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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:44 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:40 p.m.)
Indexed	1 month, 4 weeks ago (July 7, 2025, 1:25 a.m.)
Issued	27 years, 2 months ago (June 9, 1998)
Published	27 years, 2 months ago (June 9, 1998)
Published Online	27 years, 2 months ago (June 9, 1998)
Published Print	27 years, 2 months ago (June 9, 1998)

Funders 0

None

BibTeX

@article{Mitchell_1998, title={A release mechanism for stored ATP in ocular ciliary epithelial cells}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.12.7174}, DOI={10.1073/pnas.95.12.7174}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Mitchell, Claire H. and Carré, David A. and McGlinn, Alice M. and Stone, Richard A. and Civan, Mortimer M.}, year={1998}, month=jun, pages={7174–7178} }

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