DOI: 10.1073/pnas.95.12.7109. Additional evidence for an eight-transmembrane-domain topology for <i>Caenorhabditis elegans</i> and human presenilins

Additional evidence for an eight-transmembrane-domain topology for Caenorhabditis elegans and human presenilins

10.1073/pnas.95.12.7109

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Presenilins have been implicated in the genesis of Alzheimer’s disease and in facilitating LIN-12/Notch activity during development. All presenilins have multiple hydrophobic regions that could theoretically span a membrane, and a description of the membrane topology is a crucial step toward deducing the mechanism of presenilin function. Previously, we proposed an eight-transmembrane-domain model for presenilin, based on studies of the Caenorhabditis elegans SEL-12 presenilin. Here, we describe experiments that support the view that two of the hydrophobic regions of SEL-12 function as the seventh and eighth transmembrane domains. Furthermore, we have shown that human presenilin 1 behaves like SEL-12 presenilin when analyzed by our methods. Our results provide additional experimental support for the eight-transmembrane-domain model of presenilin topology.

Bibliography

Li, X., & Greenwald, I. (1998). Additional evidence for an eight-transmembrane-domain topology for Caenorhabditis elegans and human presenilins. Proceedings of the National Academy of Sciences, 95(12), 7109â7114.

Authors 2

Xiajun Li (first)
Iva Greenwald (additional)

References 36 Referenced 123

10.1006/nbdi.1996.0016
10.1038/nm0197-28
10.1038/377351a0
10.1073/pnas.94.22.12204
10.1097/00001756-199702100-00017
10.1097/00001756-199703030-00041
10.1006/bbrc.1996.6043
10.1038/375754a0
10.1074/jbc.271.49.31025
10.1073/pnas.93.25.14940
10.1016/S0092-8674(00)80244-5
10.1038/387288a0
10.1016/S0896-6273(00)80231-7
10.1016/S0896-6273(00)80232-9
10.1074/jbc.272.6.3590
10.1074/jbc.272.18.12047
10.1073/pnas.94.25.14025
10.1126/science.271.5246.159
10.1097/00001756-199705260-00054
10.1093/genetics/77.1.71
10.1016/0378-1119(90)90224-F
10.1046/j.1365-4624.1997.00012.x
10.1002/j.1460-2075.1991.tb04966.x
10.1016/1050-3862(92)90042-4
10.1016/0022-2836(82)90515-0
10.1128/mr.49.4.398-418.1985
10.1016/0022-2836(88)90539-6
10.1007/BF00233047
10.1073/pnas.92.16.7425
10.1083/jcb.133.5.1071
10.1016/S0896-6273(00)80291-3
10.1016/S0021-9258(19)61980-0
10.1073/pnas.94.18.9926
10.1126/science.277.5324.373
10.1073/pnas.94.25.14031
H A Rohan de Silva, A J Patel NeuroReport 8, i–xii (1997). / NeuroReport by Rohan de Silva H A (1997)

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:34 p.m.)
Indexed	2 months, 3 weeks ago (June 10, 2025, 2:44 p.m.)
Issued	27 years, 2 months ago (June 9, 1998)
Published	27 years, 2 months ago (June 9, 1998)
Published Online	27 years, 2 months ago (June 9, 1998)
Published Print	27 years, 2 months ago (June 9, 1998)

Funders 0

None

BibTeX

@article{Li_1998, title={Additional evidence for an eight-transmembrane-domain topology for Caenorhabditis elegans and human presenilins}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.12.7109}, DOI={10.1073/pnas.95.12.7109}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Li, Xiajun and Greenwald, Iva}, year={1998}, month=jun, pages={7109–7114} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        6,
        10
      ]
    ],
    "date-time": "2025-06-10T18:44:28Z",
    "timestamp": 1749581068757
  },
  "reference-count": 36,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "12",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1998,
        6,
        9
      ]
    ]
  },
  "abstract": "<jats:p>\n            Presenilins have been implicated in the genesis of Alzheimer\u2019s disease and in facilitating LIN-12/Notch activity during development. All presenilins have multiple hydrophobic regions that could theoretically span a membrane, and a description of the membrane topology is a crucial step toward deducing the mechanism of presenilin function. Previously, we proposed an eight-transmembrane-domain model for presenilin, based on studies of the\n            <jats:italic>Caenorhabditis elegans</jats:italic>\n            SEL-12 presenilin. Here, we describe experiments that support the view that two of the hydrophobic regions of SEL-12 function as the seventh and eighth transmembrane domains. Furthermore, we have shown that human presenilin 1 behaves like SEL-12 presenilin when analyzed by our methods. Our results provide additional experimental support for the eight-transmembrane-domain model of presenilin topology.\n          </jats:p>",
  "DOI": "10.1073/pnas.95.12.7109",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        26
      ]
    ],
    "date-time": "2002-07-26T14:40:11Z",
    "timestamp": 1027694411000
  },
  "page": "7109-7114",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 123,
  "title": "Additional evidence for an eight-transmembrane-domain topology for\n            <i>Caenorhabditis elegans</i>\n            and human presenilins",
  "prefix": "10.1073",
  "volume": "95",
  "author": [
    {
      "given": "Xiajun",
      "family": "Li",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Integrated Program in Cellular, Molecular and Biophysical Studies, Department of Biochemistry and Molecular Biophysics, and Howard Hughes Medical Institute, Columbia University College of Physicians and Surgeons, New York, NY 10032"
        }
      ]
    },
    {
      "given": "Iva",
      "family": "Greenwald",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Integrated Program in Cellular, Molecular and Biophysical Studies, Department of Biochemistry and Molecular Biophysics, and Howard Hughes Medical Institute, Columbia University College of Physicians and Surgeons, New York, NY 10032"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1998,
        6,
        9
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_3_2_1_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/nbdi.1996.0016"
    },
    {
      "key": "e_1_3_2_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nm0197-28"
    },
    {
      "key": "e_1_3_2_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/377351a0"
    },
    {
      "key": "e_1_3_2_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.94.22.12204"
    },
    {
      "key": "e_1_3_2_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1097/00001756-199702100-00017"
    },
    {
      "key": "e_1_3_2_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1097/00001756-199703030-00041"
    },
    {
      "key": "e_1_3_2_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/bbrc.1996.6043"
    },
    {
      "key": "e_1_3_2_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/375754a0"
    },
    {
      "key": "e_1_3_2_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.271.49.31025"
    },
    {
      "key": "e_1_3_2_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.93.25.14940"
    },
    {
      "key": "e_1_3_2_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80244-5"
    },
    {
      "key": "e_1_3_2_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/387288a0"
    },
    {
      "key": "e_1_3_2_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0896-6273(00)80231-7"
    },
    {
      "key": "e_1_3_2_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0896-6273(00)80232-9"
    },
    {
      "key": "e_1_3_2_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.272.6.3590"
    },
    {
      "key": "e_1_3_2_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.272.18.12047"
    },
    {
      "key": "e_1_3_2_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.94.25.14025"
    },
    {
      "key": "e_1_3_2_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.271.5246.159"
    },
    {
      "key": "e_1_3_2_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1097/00001756-199705260-00054"
    },
    {
      "key": "e_1_3_2_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/genetics/77.1.71"
    },
    {
      "key": "e_1_3_2_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0378-1119(90)90224-F"
    },
    {
      "key": "e_1_3_2_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-4624.1997.00012.x"
    },
    {
      "key": "e_1_3_2_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1991.tb04966.x"
    },
    {
      "key": "e_1_3_2_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/1050-3862(92)90042-4"
    },
    {
      "key": "e_1_3_2_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(82)90515-0"
    },
    {
      "key": "e_1_3_2_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mr.49.4.398-418.1985"
    },
    {
      "key": "e_1_3_2_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(88)90539-6"
    },
    {
      "key": "e_1_3_2_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00233047"
    },
    {
      "key": "e_1_3_2_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.92.16.7425"
    },
    {
      "key": "e_1_3_2_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.133.5.1071"
    },
    {
      "key": "e_1_3_2_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0896-6273(00)80291-3"
    },
    {
      "key": "e_1_3_2_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)61980-0"
    },
    {
      "key": "e_1_3_2_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.94.18.9926"
    },
    {
      "key": "e_1_3_2_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.277.5324.373"
    },
    {
      "key": "e_1_3_2_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.94.25.14031"
    },
    {
      "key": "e_1_3_2_36_2",
      "first-page": "i",
      "volume": "8",
      "author": "Rohan de Silva H A",
      "year": "1997",
      "unstructured": "H A Rohan de Silva, A J Patel NeuroReport 8, i\u2013xii (1997).",
      "journal-title": "NeuroReport"
    }
  ],
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.95.12.7109",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T19:34:44Z",
    "timestamp": 1649878484000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.95.12.7109"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1998,
        6,
        9
      ]
    ]
  },
  "references-count": 36,
  "journal-issue": {
    "issue": "12",
    "published-print": {
      "date-parts": [
        [
          1998,
          6,
          9
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.95.12.7109"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.95.12.7109",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1998,
        6,
        9
      ]
    ]
  },
  "assertion": [
    {
      "value": "1998-02-12",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "1998-06-09",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}