DOI: 10.1073/pnas.95.11.6474. The effect of recombinant recoverin on the photoresponse of truncated rod photoreceptors

The effect of recombinant recoverin on the photoresponse of truncated rod photoreceptors

10.1073/pnas.95.11.6474

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Recoverin is a heterogeneously acylated calcium-binding protein thought to regulate visual transduction. Its effect on the photoresponse was investigated by dialyzing the recombinant protein into truncated salamander rod outer segments. At high Ca 2+ (Ca), myristoylated recoverin (Ca-recoverin) prolonged the recovery phase of the bright flash response but had less effect on the dim flash response. The prolongation of recovery had an apparent K d for Ca of 13 μM and a Hill coefficient of 2. The prolongation was shown to be mediated by inhibition of rhodopsin deactivation. After a sudden imposed drop in Ca concentration, the effect of recoverin switched off with little lag. The myristoyl (C14:0) modification of recoverin increased its activity 12-fold, and the C12:0 or C14:2 acyl group gave similar effects. These experiments support the notion that recoverin mediates Ca-dependent inhibition of rhodopsin phosphorylation and thereby controls light-triggered phosphodiesterase activity, particularly at high light levels.

Bibliography

Erickson, M. A., Lagnado, L., Zozulya, S., Neubert, T. A., Stryer, L., & Baylor, D. A. (1998). The effect of recombinant recoverin on the photoresponse of truncated rod photoreceptors. Proceedings of the National Academy of Sciences, 95(11), 6474â6479.

Authors 6

Martha A. Erickson (first)
Leon Lagnado (additional)
Sergey Zozulya (additional)
Thomas A. Neubert (additional)
Lubert Stryer (additional)
Denis A. Baylor (additional)

References 45 Referenced 59

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:01 p.m.)
Indexed	3 months, 4 weeks ago (May 2, 2025, 5 a.m.)
Issued	27 years, 3 months ago (May 26, 1998)
Published	27 years, 3 months ago (May 26, 1998)
Published Online	27 years, 3 months ago (May 26, 1998)
Published Print	27 years, 3 months ago (May 26, 1998)

Funders 0

None

BibTeX

@article{Erickson_1998, title={The effect of recombinant recoverin on the photoresponse of truncated rod photoreceptors}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.11.6474}, DOI={10.1073/pnas.95.11.6474}, number={11}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Erickson, Martha A. and Lagnado, Leon and Zozulya, Sergey and Neubert, Thomas A. and Stryer, Lubert and Baylor, Denis A.}, year={1998}, month=may, pages={6474–6479} }

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