DOI: 10.1073/pnas.95.11.6175. Transcytosis of α <sub>1</sub> -acidic glycoprotein in the continuous microvascular endothelium

Transcytosis of α 1 -acidic glycoprotein in the continuous microvascular endothelium

10.1073/pnas.95.11.6175

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

By using perfusions and bolus administration, coupled with postembedding immunocytochemical procedures, we have identified the structures involved in the transport of derivatized orosomucoid (α 1 -acidic glycoprotein) across the continuous microvascular endothelium of the murine myocardium. Our findings indicate that: ( i ) monomeric orosomucoid binds to the luminal surface of the endothelium; ( ii ) it is restricted to caveolae during its transport across the endothelium; ( iii ) it is detected in the perivascular spaces at early time points (by 1 min) and in larger quantities at later time points (>5 min) from the beginning of its perfusion or its intravascular administration; ( iv ) no orosomucoid molecules are found in the intercellular junctions or at the abluminal exits of interendothelial spaces; and ( v ) the vesicular transport of orosomucoid is strongly inhibited by N -ethylmaleimide (>80%). Because, by size and shape, the orosomucoid qualifies as a preferential probe for the postulated small pore system, our results are discussed in relation to the pore theory of capillary permeability.

Bibliography

Predescu, D., Predescu, S., McQuistan, T., & Palade, G. E. (1998). Transcytosis of Î± 1 -acidic glycoprotein in the continuous microvascular endothelium. Proceedings of the National Academy of Sciences, 95(11), 6175â6180.

Authors 4

D. Predescu (first)
S. Predescu (additional)
T. McQuistan (additional)
G. E. Palade (additional)

References 38 Referenced 64

10.1016/0006-291X(84)91599-7
10.1016/B978-0-12-568401-9.50011-6
K Schmid Alpha1-Acid Glycoprotein: Genetics, Biochemistry, Physiological Functions and Pharmacology, eds P Baumann, W E Müller, C B Eap, J-P Tillement (Liss, New York), pp. 7–22 (1989). / Alpha1-Acid Glycoprotein: Genetics, Biochemistry, Physiological Functions and Pharmacology by Schmid K (1989)
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10.1111/j.1748-1716.1987.tb08047.x
10.1016/0026-2862(80)90024-2
F E Curry, J E Rutledge, J F Lentz Am J Physiol 257, H1354–H1359 (1989). / Am J Physiol by Curry F E (1989)
V Gross, P C Heinrich, D vanBerg, K Steube, T A Tran-Thi, K Decker, W G Erok Alpha1-Acid Glycoprotein: Genetics, Biochemistry, Physiological Functions and Pharmacology, eds P Baumann, W E Müller, C B Eap, J-P Tillement (Liss, New York), pp. 231–234 (1989). / Alpha1-Acid Glycoprotein: Genetics, Biochemistry, Physiological Functions and Pharmacology by Gross V (1989)
10.1073/pnas.91.8.3014
10.1152/ajpcell.1997.272.3.C937
D Predescu, G E Palade Am J Physiol 265, H725–H733 (1993). / Am J Physiol by Predescu D (1993)
J Q Griffith, E J Farris, A B E Roberts The Rat in Laboratory Investigation, eds J Q Griffith, E J Farris (Lippincott, Philadelphia), pp. 274–290 (1942). / The Rat in Laboratory Investigation by Griffith J Q (1942)
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10.1016/0003-2697(85)90442-7
10.1016/0003-2697(82)90113-0
J E Schnitzer, E Pinney Am J Physiol 263, H48–H55 (1992). / Am J Physiol by Schnitzer J E (1992)
G E Palade, M Simionescu, N Simionescu Acta Physiol Scand Suppl 436, 11–32 (1979). / Acta Physiol Scand Suppl by Palade G E (1979)
10.1152/physrev.1983.63.4.1536
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10.1126/science.279.5350.573
S Predescu, D Predescu, G E Palade Mol Biol Cell 8, 232a, (abstr.). (1997). / Mol Biol Cell by Predescu S (1997)
M Simionescu, N Simionescu Cell Biol Rev 25, 1–80 (1991). / Cell Biol Rev by Simionescu M (1991)
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10.1152/physrev.1994.74.1.163
10.1164/ajrccm/146.5_Pt_2.S32

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:58 p.m.)
Indexed	3 months, 2 weeks ago (May 20, 2025, 10:29 a.m.)
Issued	27 years, 3 months ago (May 26, 1998)
Published	27 years, 3 months ago (May 26, 1998)
Published Online	27 years, 3 months ago (May 26, 1998)
Published Print	27 years, 3 months ago (May 26, 1998)

Funders 0

None

BibTeX

@article{Predescu_1998, title={Transcytosis of α 1 -acidic glycoprotein in the continuous microvascular endothelium}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.11.6175}, DOI={10.1073/pnas.95.11.6175}, number={11}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Predescu, D. and Predescu, S. and McQuistan, T. and Palade, G. E.}, year={1998}, month=may, pages={6175–6180} }

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