DOI: 10.1073/pnas.95.1.316. Stoichiometric coupling of brain glucose metabolism and glutamatergic neuronal activity

Stoichiometric coupling of brain glucose metabolism and glutamatergic neuronal activity

10.1073/pnas.95.1.316

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

To determine the relationship between cerebral Glc metabolism and glutamatergic neuronal function, we used 13 C NMR spectroscopy to measure, simultaneously, the rates of the tricarboxylic acid cycle and Gln synthesis in the rat cortex in vivo . From these measurements, we calculated the rates of oxidative Glc metabolism and glutamate–neurotransmitter cycling between neurons and astrocytes (a quantitative measure of glutamatergic neuronal activity). By measuring the rates of the tricarboxylic acid cycle and Gln synthesis over a range of synaptic activity, we have determined the stoichiometry between oxidative Glc metabolism and glutamate–neurotransmitter cycling in the cortex to be close to 1:1. This finding indicates that the majority of cortical energy production supports functional (synaptic) glutamatergic neuronal activity. Another implication of this result is that brain activation studies, which map cortical oxidative Glc metabolism, provide a quantitative measure of synaptic glutamate release.

Bibliography

Sibson, N. R., Dhankhar, A., Mason, G. F., Rothman, D. L., Behar, K. L., & Shulman, R. G. (1998). Stoichiometric coupling of brain glucose metabolism and glutamatergic neuronalâactivity. Proceedings of the National Academy of Sciences, 95(1), 316â321.

Authors 6

Nicola R. Sibson (first)
Ajay Dhankhar (additional)
Graeme F. Mason (additional)
Douglas L. Rothman (additional)
Kevin L. Behar (additional)
Robert G. Shulman (additional)

References 32 Referenced 701

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S A Glantz, B K Slinker Primer of Applied Regression and Analysis of Variance (McGraw–Hill, New York, 1990). / Primer of Applied Regression and Analysis of Variance by Glantz S A (1990)
O D Creutzfeldt Brain Work: The Coupling of Function, Metabolism and Blood Flow in the Brain, Alfred Benzon Symposium VIII, eds D H Ingvar, N A Lassen (Munksgaard, Copenhagen), pp. 21–46 (1975). / Brain Work: The Coupling of Function, Metabolism and Blood Flow in the Brain, Alfred Benzon Symposium VIII by Creutzfeldt O D (1975)
A Gjedde Functional Organisation of the Human Visual Cortex, Wenner–Gren International Symposium Series, eds B Gulyás, D Ottoson, P E Roland (Pergamon, Oxford) 61, 291–306 (1993). (10.1016/B978-0-08-042004-2.50025-3) / Functional Organisation of the Human Visual Cortex, Wenner–Gren International Symposium Series by Gjedde A (1993)
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10.1111/j.1471-4159.1974.tb12227.x

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:35 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:44 p.m.)
Indexed	5 days, 4 hours ago (Aug. 28, 2025, 8:27 a.m.)
Issued	27 years, 7 months ago (Jan. 6, 1998)
Published	27 years, 7 months ago (Jan. 6, 1998)
Published Online	27 years, 7 months ago (Jan. 6, 1998)
Published Print	27 years, 7 months ago (Jan. 6, 1998)

Funders 0

None

BibTeX

@article{Sibson_1998, title={Stoichiometric coupling of brain glucose metabolism and glutamatergic neuronal activity}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.1.316}, DOI={10.1073/pnas.95.1.316}, number={1}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Sibson, Nicola R. and Dhankhar, Ajay and Mason, Graeme F. and Rothman, Douglas L. and Behar, Kevin L. and Shulman, Robert G.}, year={1998}, month=jan, pages={316–321} }

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