DOI: 10.1073/pnas.95.1.258. A chemokine expressed in lymphoid high endothelial venules promotes the adhesion and chemotaxis of naive T lymphocytes

A chemokine expressed in lymphoid high endothelial venules promotes the adhesion and chemotaxis of naive T lymphocytes

10.1073/pnas.95.1.258

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Preferential homing of naive lymphocytes to secondary lymphoid organs is thought to involve the action of chemokines, yet no chemokine has been shown to have either the expression pattern or the activities required to mediate this process. Here we show that a chemokine represented in the EST database, secondary lymphoid-tissue chemokine (SLC), is expressed in the high endothelial venules of lymph nodes and Peyer’s patches, in the T cell areas of spleen, lymph nodes, and Peyer’s patches, and in the lymphatic endothelium of multiple organs. SLC is a highly efficacious chemoattractant for lymphocytes with preferential activity toward naive T cells. Moreover, SLC induces firm adhesion of naive T lymphocytes via β2 integrin binding to the counter receptor, intercellular adhesion molecule-1, a necessary step for lymphocyte recruitment. SLC is the first chemokine demonstrated to have the characteristics required to mediate homing of lymphocytes to secondary lymphoid organs. In addition, the expression of SLC in lymphatic endothelium suggests that the migration of lymphocytes from tissues into efferent lymphatics may be an active process mediated by this molecule.

Bibliography

Gunn, M. D., Tangemann, K., Tam, C., Cyster, J. G., Rosen, S. D., & Williams, L. T. (1998). A chemokine expressed in lymphoid high endothelial venules promotes the adhesion and chemotaxis of naive Tâlymphocytes. Proceedings of the National Academy of Sciences, 95(1), 258â263.

Authors 6

Michael D. Gunn (first)
Kirsten Tangemann (additional)
Carmen Tam (additional)
Jason G. Cyster (additional)
Steven D. Rosen (additional)
Lewis T. Williams (additional)

References 38 Referenced 771

10.1016/0167-5699(95)80023-9
10.1007/BF02002614
10.1146/annurev.ph.57.030195.004143
10.1126/science.272.5258.60
10.1016/0092-8674(91)90279-8
10.1016/0955-0674(94)90092-2
10.1084/jem.178.2.497
A Hamann, D Jablonski-Westrich, A Duijvestijn, E Butcher, H Baisch, R Harder, H Thiele J Immunol 140, 693–699 (1988). (10.4049/jimmunol.140.3.693) / J Immunol by Hamann A (1988)
10.1073/pnas.89.17.8254
10.1016/0092-8674(93)90305-A
10.1146/annurev.iy.09.040191.003153
10.1146/annurev.immunol.15.1.675
K Vaddi, R C Newton J Immunol 153, 4721–4732 (1994). (10.4049/jimmunol.153.10.4721) / J Immunol by Vaddi K (1994)
10.1038/361079a0
10.1126/science.1718038
10.1084/jem.178.1.367
G Spangrude, B Braaten, R Daynes J Immunol 132, 354–362 (1984). (10.4049/jimmunol.132.1.354) / J Immunol by Spangrude G (1984)
10.1084/jem.182.2.581
10.1016/S1074-7613(00)80682-2
10.1038/42716
10.1074/jbc.272.31.19518
10.1074/jbc.272.21.13803
J Hedrick, A Zlotnik J Immunol 159, 1589–1593 (1997). (10.4049/jimmunol.159.4.1589) / J Immunol by Hedrick J (1997)
R Hromas, C Kim, M Klemsz, M Krathwohl, K Fife, S Cooper, C Schnizlein-Bick, H Broxmeyer J Immunol 159, 2554–2558 (1997). (10.4049/jimmunol.159.6.2554) / J Immunol by Hromas R (1997)
10.1084/jem.184.3.1101
R Budd, J Cerottini, C Horvath, C Bron, T Pedrazzini, R Howe, H MacDonald J Immunol 138, 3120–3129 (1987). (10.4049/jimmunol.138.10.3120) / J Immunol by Budd R (1987)
T Jung, W Gallatin, I Weissman, M Dailey J Immunol 141, 4110–4117 (1988). (10.4049/jimmunol.141.12.4110) / J Immunol by Jung T (1988)
A Lloyd, J Oppenheim, D Kelvin, D Taub J Immunol 156, 932–938 (1996). (10.4049/jimmunol.156.3.932) / J Immunol by Lloyd A (1996)
10.1084/jem.184.4.1343
10.1016/S0065-2776(08)60501-5
10.1016/0167-5699(96)80545-7
M Williams, E Butcher J Immunol 159, 1746–1752 (1997). (10.4049/jimmunol.159.4.1746) / J Immunol by Williams M (1997)
10.1016/S0960-9822(06)00105-9
10.1016/S0092-8674(00)81798-5
10.1016/0167-5699(92)90039-A
10.1073/pnas.85.8.2444
10.1006/geno.1996.0177
M D Adams, A R Kerlavage, R D Fleischmann, R A Fuldner, C J Bult, N H Lee, E F Kirkness, K G Weinstock, J D Gocayne, O White, et al. Nature (London) 377, 3–174 (1995). / Nature (London) by Adams M D (1995)

Dates

Type	When
Created	23 years ago (July 26, 2002, 10:40 a.m.)
Deposited	2 years, 4 months ago (April 22, 2023, 8:25 a.m.)
Indexed	1 month, 2 weeks ago (July 8, 2025, 11:29 a.m.)
Issued	27 years, 7 months ago (Jan. 6, 1998)
Published	27 years, 7 months ago (Jan. 6, 1998)
Published Online	27 years, 7 months ago (Jan. 6, 1998)
Published Print	27 years, 7 months ago (Jan. 6, 1998)

Funders 0

None

BibTeX

@article{Gunn_1998, title={A chemokine expressed in lymphoid high endothelial venules promotes the adhesion and chemotaxis of naive T lymphocytes}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.1.258}, DOI={10.1073/pnas.95.1.258}, number={1}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Gunn, Michael D. and Tangemann, Kirsten and Tam, Carmen and Cyster, Jason G. and Rosen, Steven D. and Williams, Lewis T.}, year={1998}, month=jan, pages={258–263} }

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