DOI: 10.1073/pnas.95.1.201. Control of direction of flagellar rotation in bacterial chemotaxis

Control of direction of flagellar rotation in bacterial chemotaxis

10.1073/pnas.95.1.201

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The motile behavior of the bacterium Escherichia coli depends on the direction of rotation of its flagellar motors. Binding of the phosphorylated signaling molecule CheY to a motor component FliM is known to enhance clockwise rotation. It is difficult to study this interaction in vivo , because the dynamics of phosphorylation of CheY by its kinase CheA and the hydrolysis of CheY (accelerated by CheZ) are not under direct experimental control. Here, we examine instead the interaction with the flagellar motor of a double mutant CheY 13DK106YW that is active without phosphorylation. The behavioral assays were carried out on tethered cells lacking CheA and CheZ. The effects of variation in intracellular concentration of the mutant protein were highly nonlinear. However, they can be explained by a thermal isomerization model in which the free energies of clockwise and counterclockwise states depend linearly on the amount of CheY bound.

Bibliography

Scharf, B. E., Fahrner, K. A., Turner, L., & Berg, H. C. (1998). Control of direction of flagellar rotation in bacterialâchemotaxis. Proceedings of the National Academy of Sciences, 95(1), 201â206.

Authors 4

Birgit E. Scharf (first)
Karen A. Fahrner (additional)
Linda Turner (additional)
Howard C. Berg (additional)

References 41 Referenced 194

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10.1111/j.1365-2958.1996.tb02531.x
R M Macnab Escherichia coli and Salmonella: Cellular and Molecular Biology, eds F C Neidhardt, R Curtiss, J L Ingraham, E C C Lin, K B Low, B Magasanik, W S Reznikoff, M Riley, M Schaechter, H E Umbarger (Am. Soc. Microbiol., Washington, DC), pp. 123–145 (1996). / Escherichia coli and Salmonella: Cellular and Molecular Biology by Macnab R M (1996)
J B Stock, M G Surette Escherichia coli and Salmonella: Cellular and Molecular Biology, eds F C Neidhardt, R Curtiss, J L Ingraham, E C C Lin, K B Low, B Magasanik, W S Reznikoff, M Riley, M Schaechter, H E Umbarger (Am. Soc. Microbiol., Washington, DC), pp. 1103–1129 (1996). / Escherichia coli and Salmonella: Cellular and Molecular Biology by Stock J B (1996)
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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:42 p.m.)
Indexed	2 months, 1 week ago (June 20, 2025, 8:06 a.m.)
Issued	27 years, 7 months ago (Jan. 6, 1998)
Published	27 years, 7 months ago (Jan. 6, 1998)
Published Online	27 years, 7 months ago (Jan. 6, 1998)
Published Print	27 years, 7 months ago (Jan. 6, 1998)

Funders 0

None

BibTeX

@article{Scharf_1998, title={Control of direction of flagellar rotation in bacterial chemotaxis}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.1.201}, DOI={10.1073/pnas.95.1.201}, number={1}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Scharf, Birgit E. and Fahrner, Karen A. and Turner, Linda and Berg, Howard C.}, year={1998}, month=jan, pages={201–206} }

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