DOI: 10.1073/pnas.95.1.120. Negative control of bacterial DNA replication by a cell cycle regulatory protein that binds at the chromosome origin

Negative control of bacterial DNA replication by a cell cycle regulatory protein that binds at the chromosome origin

10.1073/pnas.95.1.120

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Caulobacter crescentus divides asymmetrically generating two distinct cell types at each cell division: a stalked cell competent for DNA replication, and a swarmer cell that is unable to initiate DNA replication until it differentiates into a stalked cell later in the cell cycle. The CtrA protein, a member of the response regulator family of the two-component signal transduction system, controls multiple cell cycle processes in Caulobacter and is present in swarmer cells but absent from stalked cells. We report that CtrA binds five sites within the chromosome replication origin in vitro . These sites overlap an essential DnaA box and a promoter in the origin that is essential for replication initiation. Analysis of mutant alleles of ctrA and point mutations in one of the CtrA binding sites in the origin demonstrate that CtrA represses replication in vivo . CtrA-mediated repression at the origin thus restricts replication to the stalked cell type. Thus, the direct coupling of chromosome replication with the cell cycle is mediated by the ubiquitous two-component signaling proteins.

Bibliography

Quon, K. C., Yang, B., Domian, I. J., Shapiro, L., & Marczynski, G. T. (1998). Negative control of bacterial DNA replication by a cell cycle regulatory protein that binds at the chromosomeâorigin. Proceedings of the National Academy of Sciences, 95(1), 120â125.

Authors 5

Kim C. Quon (first)
Bing Yang (additional)
Ibrahim J. Domian (additional)
Lucy Shapiro (additional)
Gregory T. Marczynski (additional)

References 36 Referenced 294

Dates

Type	When
Created	23 years ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:43 p.m.)
Indexed	5 days, 20 hours ago (Aug. 19, 2025, 6:17 a.m.)
Issued	27 years, 7 months ago (Jan. 6, 1998)
Published	27 years, 7 months ago (Jan. 6, 1998)
Published Online	27 years, 7 months ago (Jan. 6, 1998)
Published Print	27 years, 7 months ago (Jan. 6, 1998)

Funders 0

None

BibTeX

@article{Quon_1998, title={Negative control of bacterial DNA replication by a cell cycle regulatory protein that binds at the chromosome origin}, volume={95}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.95.1.120}, DOI={10.1073/pnas.95.1.120}, number={1}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Quon, Kim C. and Yang, Bing and Domian, Ibrahim J. and Shapiro, Lucy and Marczynski, Gregory T.}, year={1998}, month=jan, pages={120–125} }

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  "abstract": "<jats:p>\n            <jats:italic>Caulobacter crescentus</jats:italic>\n            divides asymmetrically generating two distinct cell types at each cell division: a stalked cell competent for DNA replication, and a swarmer cell that is unable to initiate DNA replication until it differentiates into a stalked cell later in the cell cycle. The CtrA protein, a member of the response regulator family of the two-component signal transduction system, controls multiple cell cycle processes in\n            <jats:italic>Caulobacter</jats:italic>\n            and is present in swarmer cells but absent from stalked cells. We report that CtrA binds five sites within the chromosome replication origin\n            <jats:italic>in vitro</jats:italic>\n            . These sites overlap an essential DnaA box and a promoter in the origin that is essential for replication initiation. Analysis of mutant alleles of\n            <jats:italic>ctrA</jats:italic>\n            and point mutations in one of the CtrA binding sites in the origin demonstrate that CtrA represses replication\n            <jats:italic>in vivo</jats:italic>\n            . CtrA-mediated repression at the origin thus restricts replication to the stalked cell type. Thus, the direct coupling of chromosome replication with the cell cycle is mediated by the ubiquitous two-component signaling proteins.\n          </jats:p>",
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