DOI: 10.1073/pnas.94.9.4401. Functional and idling rotatory motion within F <sub>1</sub> -ATPase

Functional and idling rotatory motion within F 1 -ATPase

10.1073/pnas.94.9.4401

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

ATP synthase mediates proton flow through its membrane portion, F 0 , which drives the synthesis of ATP in its headpiece, F 1 . The F 1 -portion contains a hexagonal array of three subunits α and three β encircling a central subunit γ, that in turn interacts with a smaller ɛ and with F 0 . Recently we reported that the application of polarized absorption recovery after photobleaching showed the ATP-driven rotation of γ over at least two, if not three, β. Here we extend probes of such rotation aided by a new theory for assessing continuous versus stepped, Brownian versus unidirectional molecular motion. The observed relaxation of the absorption anisotropy is fully compatible with a unidirectional and stepping rotation of γ over three equidistantly spaced angular positions in the hexagon formed by the alternating subunits α and β. The results strongly support a rotational catalysis with equal participation of all three catalytic sites. In addition we report a limited rotation of γ without added nucleotides, perhaps idling and of Brownian nature, that covers only a narrow angular domain.

Bibliography

Sabbert, D., Engelbrecht, S., & Junge, W. (1997). Functional and idling rotatory motion withinâF 1 -ATPase. Proceedings of the National Academy of Sciences, 94(9), 4401â4405.

Authors 3

D. Sabbert (first)
S. Engelbrecht (additional)
W. Junge (additional)

References 45 Referenced 74

P Mitchell Physiol Rev 41, 445–502 (1966). / Physiol Rev by Mitchell P (1966)
10.1152/physrev.1988.68.1.177
J E Walker The Biochemist 16, 31–351 (1994). / The Biochemist by Walker J E (1994)
10.1016/S0167-7306(08)60181-7
10.1016/S0959-440X(96)80114-X
10.1042/bst0230747
10.1016/0005-2728(93)90112-S
10.1016/0005-2728(94)90009-4
10.1016/0005-2728(94)90184-8
10.1021/bi00475a008
10.1111/j.1432-1033.1994.tb18904.x
10.1016/S0021-9258(18)67209-6
10.1038/370621a0
10.1016/S0021-9258(20)80703-0
10.1073/pnas.70.10.2837
P D Boyer, W E Kohlbrenner Energy Coupling in Photosynthesis, eds B R Selman, S Selman-Reimer (Elsevier, New York), pp. 231–241 (1981). / Energy Coupling in Photosynthesis by Boyer P D (1981)
10.1016/0005-2728(93)90063-L
10.1016/S0005-2728(05)80099-4
10.1007/BF01871046
10.1006/jmbi.1995.0586
10.1073/pnas.87.24.9585
10.1016/0005-2728(87)90073-9
10.1073/pnas.92.24.10964
10.1038/381623a0
10.1016/S0021-9258(17)39869-1
10.1016/0014-5793(88)80421-6
10.1111/j.1432-1033.1986.tb10085.x
10.1021/bi00537a029
10.1016/0005-2728(77)90190-6
10.1016/B978-0-12-511050-1.50053-3
10.1111/j.1432-1033.1990.tb15476.x
10.1016/0014-5793(77)80492-4
10.1007/BF01341525
10.1021/bi00525a003
10.1021/bi00465a014
10.1016/S0006-3495(77)85550-1
10.1074/jbc.271.5.2433
10.1021/bi00083a008
10.1016/0005-2728(96)00004-7
10.1016/0301-4622(84)87020-9
10.1016/0301-0104(75)87003-0
10.1073/pnas.93.6.2312
10.1074/jbc.270.21.12653
10.1016/0005-2736(91)90114-N
10.1038/386299a0

Dates

Type	When
Created	23 years ago (July 26, 2002, 10:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:58 p.m.)
Indexed	2 weeks, 4 days ago (Aug. 6, 2025, 9:21 a.m.)
Issued	28 years, 3 months ago (April 29, 1997)
Published	28 years, 3 months ago (April 29, 1997)
Published Online	28 years, 3 months ago (April 29, 1997)
Published Print	28 years, 3 months ago (April 29, 1997)

Funders 0

None

BibTeX

@article{Sabbert_1997, title={Functional and idling rotatory motion within F 1 -ATPase}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.9.4401}, DOI={10.1073/pnas.94.9.4401}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Sabbert, D. and Engelbrecht, S. and Junge, W.}, year={1997}, month=apr, pages={4401–4405} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        6
      ]
    ],
    "date-time": "2025-08-06T13:21:29Z",
    "timestamp": 1754486489804
  },
  "reference-count": 45,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "9",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1997,
        4,
        29
      ]
    ]
  },
  "abstract": "<jats:p>\n            ATP synthase mediates proton flow through its membrane portion, F\n            <jats:sub>0</jats:sub>\n            , which drives the synthesis of ATP in its headpiece, F\n            <jats:sub>1</jats:sub>\n            . The F\n            <jats:sub>1</jats:sub>\n            -portion contains a hexagonal array of three subunits \u03b1 and three \u03b2 encircling a central subunit \u03b3, that in turn interacts with a smaller \u025b and with F\n            <jats:sub>0</jats:sub>\n            . Recently we reported that the application of polarized absorption recovery after photobleaching showed the ATP-driven rotation of \u03b3 over at least two, if not three, \u03b2. Here we extend probes of such rotation aided by a new theory for assessing continuous versus stepped, Brownian versus unidirectional molecular motion. The observed relaxation of the absorption anisotropy is fully compatible with a unidirectional and stepping rotation of \u03b3 over three equidistantly spaced angular positions in the hexagon formed by the alternating subunits \u03b1 and \u03b2. The results strongly support a rotational catalysis with equal participation of all three catalytic sites. In addition we report a limited rotation of \u03b3 without added nucleotides, perhaps idling and of Brownian nature, that covers only a narrow angular domain.\n          </jats:p>",
  "DOI": "10.1073/pnas.94.9.4401",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        26
      ]
    ],
    "date-time": "2002-07-26T14:40:52Z",
    "timestamp": 1027694452000
  },
  "page": "4401-4405",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 74,
  "title": "Functional and idling rotatory motion within\u2009F\n            <sub>1</sub>\n            -ATPase",
  "prefix": "10.1073",
  "volume": "94",
  "author": [
    {
      "given": "D.",
      "family": "Sabbert",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Abteilung Biophysik, Fachbereich Biologie/Chemie, Universit\u00e4t Osnabr\u00fcck, D-49069 Osnabr\u00fcck, Germany"
        }
      ]
    },
    {
      "given": "S.",
      "family": "Engelbrecht",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Abteilung Biophysik, Fachbereich Biologie/Chemie, Universit\u00e4t Osnabr\u00fcck, D-49069 Osnabr\u00fcck, Germany"
        }
      ]
    },
    {
      "given": "W.",
      "family": "Junge",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Abteilung Biophysik, Fachbereich Biologie/Chemie, Universit\u00e4t Osnabr\u00fcck, D-49069 Osnabr\u00fcck, Germany"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1997,
        4,
        29
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_3_4_1_2",
      "first-page": "445",
      "volume": "41",
      "author": "Mitchell P",
      "year": "1966",
      "unstructured": "P Mitchell Physiol Rev 41, 445\u2013502 (1966).",
      "journal-title": "Physiol Rev"
    },
    {
      "key": "e_1_3_4_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1152/physrev.1988.68.1.177"
    },
    {
      "key": "e_1_3_4_3_2",
      "first-page": "31",
      "volume": "16",
      "author": "Walker J E",
      "year": "1994",
      "unstructured": "J E Walker The Biochemist 16, 31\u2013351 (1994).",
      "journal-title": "The Biochemist"
    },
    {
      "key": "e_1_3_4_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0167-7306(08)60181-7"
    },
    {
      "key": "e_1_3_4_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0959-440X(96)80114-X"
    },
    {
      "key": "e_1_3_4_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bst0230747"
    },
    {
      "key": "e_1_3_4_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(93)90112-S"
    },
    {
      "key": "e_1_3_4_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(94)90009-4"
    },
    {
      "key": "e_1_3_4_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(94)90184-8"
    },
    {
      "key": "e_1_3_4_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00475a008"
    },
    {
      "key": "e_1_3_4_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1994.tb18904.x"
    },
    {
      "key": "e_1_3_4_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)67209-6"
    },
    {
      "key": "e_1_3_4_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/370621a0"
    },
    {
      "key": "e_1_3_4_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(20)80703-0"
    },
    {
      "key": "e_1_3_4_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.70.10.2837"
    },
    {
      "key": "e_1_3_4_16_2",
      "first-page": "231",
      "volume-title": "Energy Coupling in Photosynthesis",
      "author": "Boyer P D",
      "year": "1981",
      "unstructured": "P D Boyer, W E Kohlbrenner Energy Coupling in Photosynthesis, eds B R Selman, S Selman-Reimer (Elsevier, New York), pp. 231\u2013241 (1981)."
    },
    {
      "key": "e_1_3_4_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(93)90063-L"
    },
    {
      "key": "e_1_3_4_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0005-2728(05)80099-4"
    },
    {
      "key": "e_1_3_4_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01871046"
    },
    {
      "key": "e_1_3_4_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jmbi.1995.0586"
    },
    {
      "key": "e_1_3_4_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.87.24.9585"
    },
    {
      "key": "e_1_3_4_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(87)90073-9"
    },
    {
      "key": "e_1_3_4_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.92.24.10964"
    },
    {
      "key": "e_1_3_4_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/381623a0"
    },
    {
      "key": "e_1_3_4_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(17)39869-1"
    },
    {
      "key": "e_1_3_4_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(88)80421-6"
    },
    {
      "key": "e_1_3_4_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1986.tb10085.x"
    },
    {
      "key": "e_1_3_4_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00537a029"
    },
    {
      "key": "e_1_3_4_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(77)90190-6"
    },
    {
      "key": "e_1_3_4_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/B978-0-12-511050-1.50053-3"
    },
    {
      "key": "e_1_3_4_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1990.tb15476.x"
    },
    {
      "key": "e_1_3_4_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(77)80492-4"
    },
    {
      "key": "e_1_3_4_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01341525"
    },
    {
      "key": "e_1_3_4_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00525a003"
    },
    {
      "key": "e_1_3_4_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00465a014"
    },
    {
      "key": "e_1_3_4_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0006-3495(77)85550-1"
    },
    {
      "key": "e_1_3_4_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.271.5.2433"
    },
    {
      "key": "e_1_3_4_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00083a008"
    },
    {
      "key": "e_1_3_4_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(96)00004-7"
    },
    {
      "key": "e_1_3_4_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0301-4622(84)87020-9"
    },
    {
      "key": "e_1_3_4_41_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0301-0104(75)87003-0"
    },
    {
      "key": "e_1_3_4_42_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.93.6.2312"
    },
    {
      "key": "e_1_3_4_43_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.270.21.12653"
    },
    {
      "key": "e_1_3_4_44_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2736(91)90114-N"
    },
    {
      "key": "e_1_3_4_45_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/386299a0"
    }
  ],
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.94.9.4401",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T19:58:52Z",
    "timestamp": 1649879932000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.94.9.4401"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1997,
        4,
        29
      ]
    ]
  },
  "references-count": 45,
  "journal-issue": {
    "issue": "9",
    "published-print": {
      "date-parts": [
        [
          1997,
          4,
          29
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.94.9.4401"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.94.9.4401",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1997,
        4,
        29
      ]
    ]
  },
  "assertion": [
    {
      "value": "1996-12-28",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "1997-02-28",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "1997-04-29",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}