DOI: 10.1073/pnas.94.9.4289. Gene induction in response to unfolded protein in the endoplasmic reticulum is mediated through Ire1p kinase interaction with a transcriptional coactivator complex containing Ada5p

Gene induction in response to unfolded protein in the endoplasmic reticulum is mediated through Ire1p kinase interaction with a transcriptional coactivator complex containing Ada5p

10.1073/pnas.94.9.4289

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

In eukaryotic cells, accumulation of unfolded protein in the endoplasmic reticulum induces transcription of a family of genes encoding endoplasmic reticulum protein chaperones through a conserved unfolded protein response element. In Saccharomyces cerevisiae, activation of a transmembrane receptor kinase, Ire1p (Ern1p), initiates signaling, although the mediators immediately downstream of Ire1 kinase are unknown. Here we demonstrate interaction of Ire1p with the transcriptional coactivator, Gcn5p (for general control nonrepressed; also known as Ada4p). Gcn5p associates with other Ada (for alteration/deficiency in activation) gene products in a heteromeric complex and has histone acetyltransferase activity. We show that the Gcn5/Ada complex is selectively required for the unfolded protein response but not for the heat shock response. A novel mechanism is proposed in which activation of a receptor kinase recruits a transcription coactivator complex to a specific chromosomal locus to mediate localized histone acetylation, thus making specific gene sequences accessible for transcription.

Bibliography

Welihinda, A. A., Tirasophon, W., Green, S. R., & Kaufman, R. J. (1997). Gene induction in response to unfolded protein in the endoplasmic reticulum is mediated through Ire1p kinase interaction with a transcriptional coactivator complexâcontainingâAda5p. Proceedings of the National Academy of Sciences, 94(9), 4289â4294.

Authors 4

Ajith A. Welihinda (first)
Witoon Tirasophon (additional)
Sarah R. Green (additional)
Randal J. Kaufman (additional)

References 33 Referenced 49

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:43 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:04 p.m.)
Indexed	11 months, 3 weeks ago (Sept. 3, 2024, 11:47 a.m.)
Issued	28 years, 3 months ago (April 29, 1997)
Published	28 years, 3 months ago (April 29, 1997)
Published Online	28 years, 3 months ago (April 29, 1997)
Published Print	28 years, 3 months ago (April 29, 1997)

Funders 0

None

BibTeX

@article{Welihinda_1997, title={Gene induction in response to unfolded protein in the endoplasmic reticulum is mediated through Ire1p kinase interaction with a transcriptional coactivator complex containing Ada5p}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.9.4289}, DOI={10.1073/pnas.94.9.4289}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Welihinda, Ajith A. and Tirasophon, Witoon and Green, Sarah R. and Kaufman, Randal J.}, year={1997}, month=apr, pages={4289–4294} }

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