DOI: 10.1073/pnas.94.9.4262. A general purpose RNA-cleaving DNA enzyme

A general purpose RNA-cleaving DNA enzyme

10.1073/pnas.94.9.4262

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

An in vitro selection procedure was used to develop a DNA enzyme that can be made to cleave almost any targeted RNA substrate under simulated physiological conditions. The enzyme is comprised of a catalytic domain of 15 deoxynucleotides, flanked by two substrate-recognition domains of seven to eight deoxynucleotides each. The RNA substrate is bound through Watson–Crick base pairing and is cleaved at a particular phosphodiester located between an unpaired purine and a paired pyrimidine residue. Despite its small size, the DNA enzyme has a catalytic efficiency ( k cat / K m ) of ≈10 9 M −1 ⋅min −1 under multiple turnover conditions, exceeding that of any other known nucleic acid enzyme. Its activity is dependent on the presence of Mg 2+ ion. By changing the sequence of the substrate-recognition domains, the DNA enzyme can be made to target different RNA substrates. In this study, for example, it was directed to cleave synthetic RNAs corresponding to the start codon region of HIV-1 gag / pol , env , vpr , tat , and nef mRNAs.

Bibliography

Santoro, S. W., & Joyce, G. F. (1997). A general purpose RNA-cleaving DNAâenzyme. Proceedings of the National Academy of Sciences, 94(9), 4262â4266.

Authors 2

Stephen W. Santoro (first)
Gerald F. Joyce (additional)

References 28 Referenced 1,268

Dates

Type	When
Created	23 years ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:56 p.m.)
Indexed	2 days, 19 hours ago (Aug. 20, 2025, 8:55 a.m.)
Issued	28 years, 3 months ago (April 29, 1997)
Published	28 years, 3 months ago (April 29, 1997)
Published Online	28 years, 3 months ago (April 29, 1997)
Published Print	28 years, 3 months ago (April 29, 1997)

Funders 0

None

BibTeX

@article{Santoro_1997, title={A general purpose RNA-cleaving DNA enzyme}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.9.4262}, DOI={10.1073/pnas.94.9.4262}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Santoro, Stephen W. and Joyce, Gerald F.}, year={1997}, month=apr, pages={4262–4266} }

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