DOI: 10.1073/pnas.94.7.3151. <i>mcm5/cdc46-bob1</i> bypasses the requirement for the S phase activator Cdc7p

mcm5/cdc46-bob1 bypasses the requirement for the S phase activator Cdc7p

10.1073/pnas.94.7.3151

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Cdc7p is a protein kinase that is required for G 1 /S transition and initiation of DNA replication in Saccharomyces cerevisiae . The mechanisms whereby Cdc7p and its substrates exerts their effects are unknown. We report here the characterization in S. cerevisiae of a recessive mutation in a member of the MCM family, MCM5/ CDC46 , which bypasses the requirement for Cdc7p and its interacting factor Dbf4p. Because the MCM family of evolutionarily conserved proteins have been implicated in restricting DNA replication to once per cell cycle, our studies suggest that Cdc7p is required late in G 1 because in its absence the Mcm5p/Cdc46p blocks the initiation of DNA replication. Moreover, Mcm5p/Cdc46p may have both positive and negative effects on the ability of cell to initiate replication.

Bibliography

Hardy, C. F. J., Dryga, O., Seematter, S., Pahl, P. M. B., & Sclafani, R. A. (1997). mcm5/cdc46-bob1 bypasses the requirement for the S phase activatorâCdc7p. Proceedings of the National Academy of Sciences, 94(7), 3151â3155.

Authors 5

Christopher F. J. Hardy (first)
Olga Dryga (additional)
Sara Seematter (additional)
Paula M. B. Pahl (additional)
Robert A. Sclafani (additional)

References 40 Referenced 208

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Dates

Type	When
Created	23 years ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:32 p.m.)
Indexed	1 year ago (Aug. 19, 2024, 10:32 a.m.)
Issued	28 years, 4 months ago (April 1, 1997)
Published	28 years, 4 months ago (April 1, 1997)
Published Online	28 years, 4 months ago (April 1, 1997)
Published Print	28 years, 4 months ago (April 1, 1997)

Funders 0

None

BibTeX

@article{Hardy_1997, title={mcm5/cdc46-bob1 bypasses the requirement for the S phase activator Cdc7p}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.7.3151}, DOI={10.1073/pnas.94.7.3151}, number={7}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Hardy, Christopher F. J. and Dryga, Olga and Seematter, Sara and Pahl, Paula M. B. and Sclafani, Robert A.}, year={1997}, month=apr, pages={3151–3155} }

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