DOI: 10.1073/pnas.94.7.2987. Gene 4 helicase of bacteriophage T7 mediates strand transfer through pyrimidine dimers, mismatches, and nonhomologous regions

Gene 4 helicase of bacteriophage T7 mediates strand transfer through pyrimidine dimers, mismatches, and nonhomologous regions

10.1073/pnas.94.7.2987

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

In bacteriophage T7 the gene 2.5 single-stranded DNA-binding protein and the gene 4 helicase together promote the annealing of homologous regions of two DNA partners to form a joint molecule and subsequent strand transfer. In this reaction T7 gene 2.5 protein is essential for joint molecule formation, but is not required for T7 gene 4 protein-mediated strand transfer. T7 gene 4 helicase alone is able to mediate strand transfer, provided that a joint molecule is available. The present paper shows that, in addition, strand transfer proceeds at a normal rate even when both DNA partners contain ultraviolet-induced pyrimidine dimers (0.6 dimer per 100 nt). An insert of a relatively long (842-nt) segment of nonhomologous DNA in the single-stranded DNA partner has no effect on strand transfer, whereas its presence in the double-stranded partner prevents strand transfer. A short insert (37 nt) can be tolerated in either partner. Thus, DNA helicase is able to participate in recombinational DNA repair through its role in strand exchange, providing a pathway distinct from nucleotide excision repair.

Bibliography

Kong, D., Griffith, J. D., & Richardson, C. C. (1997). Gene 4 helicase of bacteriophage T7 mediates strand transfer through pyrimidine dimers, mismatches, and nonhomologousâregions. Proceedings of the National Academy of Sciences, 94(7), 2987â2992.

Authors 3

Daochun Kong (first)
Jack D. Griffith (additional)
Charles C. Richardson (additional)

References 40 Referenced 17

10.1016/0092-8674(95)90057-8
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E C Friedberg, G C Walker, W Siede DNA Repair and Mutagenesis (Am. Soc. Microbiol., Washington, DC), pp. 407–593 (1995). / DNA Repair and Mutagenesis by Friedberg E C (1995)
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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:32 p.m.)
Indexed	1 year, 8 months ago (Dec. 23, 2023, 2:18 a.m.)
Issued	28 years, 4 months ago (April 1, 1997)
Published	28 years, 4 months ago (April 1, 1997)
Published Online	28 years, 4 months ago (April 1, 1997)
Published Print	28 years, 4 months ago (April 1, 1997)

Funders 0

None

BibTeX

@article{Kong_1997, title={Gene 4 helicase of bacteriophage T7 mediates strand transfer through pyrimidine dimers, mismatches, and nonhomologous regions}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.7.2987}, DOI={10.1073/pnas.94.7.2987}, number={7}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kong, Daochun and Griffith, Jack D. and Richardson, Charles C.}, year={1997}, month=apr, pages={2987–2992} }

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