DOI: 10.1073/pnas.94.7.2951. Single-strand DNA intermediates in phage λ’s Red recombination pathway

Single-strand DNA intermediates in phage λ’s Red recombination pathway

10.1073/pnas.94.7.2951

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

An assay was developed to assess early intermediates arising in λ’s Red recombination pathway. Double-strand breaks were delivered in vivo to nonreplicating λ chromosomes. Analysis by blot hybridization of total DNA extracts revealed the following: ( i ) long (>1.4 kilobases) single-strand DNA (ssDNA) intermediates; ( ii ) resection proceeding bidirectionally from the break site; ( iii ) single-strand overhangs of 3′ polarity; and ( iv ) in the absence of λ’s ninR functions, a requirement of the red α gene product for the production of ssDNA. Therefore, the physical characteristics exhibited by these ssDNA molecules are consistent with their being an early recombination intermediate in the Red recombination pathway as proposed previously from genetic and in vitro biochemical analyses.

Bibliography

Hill, S. A., Stahl, M. M., & Stahl, F. W. (1997). Single-strand DNA intermediates in phage Î»âs Red recombinationâpathway. Proceedings of the National Academy of Sciences, 94(7), 2951â2956.

Authors 3

Stuart A. Hill (first)
Mary M. Stahl (additional)
Franklin W. Stahl (additional)

References 40 Referenced 22

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10.1016/S0021-9258(18)62316-6
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D L Daniels, J L Schroeder, W Szybalski, F Sanger, F R Blattner Lambda II, eds R W Hendrix, J W Roberts, F W Stahl, R A Weisberg (Cold Spring Harbor Lab. Press, Plainview, NY), pp. 469–676 (1983). / Lambda II by Daniels D L (1983)
10.1016/0022-2836(70)90416-X
J Zissler, E Signer, F Schaefer The Bacteriophage Lambda, ed A D Hersey (Cold Spring Harbor Lab. Press, Plainview, NY), pp. 455–469 (1971). / The Bacteriophage Lambda by Zissler J (1971)
10.1016/0042-6822(69)90060-9
W Arber, L Enquist, B Hohn, N E Murray, K Murray Lambda II, eds R W Hendrix, J W Roberts, F W Stahl, R A Weisberg (Cold Spring Harbor Lab. Press, Plainview, NY), pp. 433–466 (1983). / Lambda II by Arber W (1983)
F M Ausubel, R Brent, R E Kingston, D D Moore, J G Seidman, J A Smith, K Struhl Current Protocols in Molecular Biology 1 (Greene & Wiley, New York, 1989). / Current Protocols in Molecular Biology 1 by Ausubel F M (1989)
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10.1128/mr.58.3.401-465.1994
10.1128/mcb.12.2.563-575.1992
10.1093/genetics/134.4.1013
10.1038/newbio229013a0
10.1111/j.1365-2958.1994.tb00286.x
J R Gillen, A J Clark Mechanism of Recombination, ed R F Grell (Plenum, New York), pp. 123–136 (1974). (10.1007/978-1-4684-2133-0_12) / Mechanism of Recombination by Gillen J R (1974)
10.1128/jb.145.1.521-532.1981
10.1093/genetics/139.1.5
10.1128/jb.177.7.1692-1698.1995

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:35 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:29 p.m.)
Indexed	1 year, 1 month ago (July 27, 2024, 11:11 a.m.)
Issued	28 years, 5 months ago (April 1, 1997)
Published	28 years, 5 months ago (April 1, 1997)
Published Online	28 years, 5 months ago (April 1, 1997)
Published Print	28 years, 5 months ago (April 1, 1997)

Funders 0

None

BibTeX

@article{Hill_1997, title={Single-strand DNA intermediates in phage λ’s Red recombination pathway}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.7.2951}, DOI={10.1073/pnas.94.7.2951}, number={7}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Hill, Stuart A. and Stahl, Mary M. and Stahl, Franklin W.}, year={1997}, month=apr, pages={2951–2956} }

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