DOI: 10.1073/pnas.94.7.2927. CREB-binding protein/p300 are transcriptional coactivators of p65

CREB-binding protein/p300 are transcriptional coactivators of p65

10.1073/pnas.94.7.2927

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

CBP (CREB-binding protein) and p300 are versatile coactivators that link transcriptional activators to the basal transcriptional apparatus. In the present study, we identify CBP and p300 as coactivators of the nuclear factor-κB (NF-κB) component p65 (RelA). Consistent with their role as coactivators, both CBP and p300 potentiated p65-activated transcription of E-selectin and VCAM-1–CAT reporter constructs. The N- and C-terminal domains of both CBP/p300 functionally interact with a region of p65 containing the transcriptional activation domain as demonstrated by mammalian two-hybrid assays. Direct physical interactions of CBP/p300 with p65 were demonstrated by glutathioneS-transferase fusion protein binding, and coimmunoprecipitation/Western blot studies. The adenovirus E1A 12S protein, which complexes with CBP and p300, inhibited p65-dependent gene expression. Reporter gene expression could be rescued from E1A inhibition by overexpression of CBP or p300. CBP and p300 act as coactivators of p65-driven gene activation and may play an important role in the cytokine-induced expression of various immune and inflammatory genes.

Bibliography

Gerritsen, M. E., Williams, A. J., Neish, A. S., Moore, S., Shi, Y., & Collins, T. (1997). CREB-binding protein/p300 are transcriptional coactivatorsâofâp65. Proceedings of the National Academy of Sciences, 94(7), 2927â2932.

Authors 6

Mary E. Gerritsen (first)
Amy J. Williams (additional)
Andrew S. Neish (additional)
Sarah Moore (additional)
Yang Shi (additional)
Tucker Collins (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	2 years, 4 months ago (April 22, 2023, 8:10 a.m.)
Indexed	2 days, 4 hours ago (Aug. 28, 2025, 8:04 a.m.)
Issued	28 years, 4 months ago (April 1, 1997)
Published	28 years, 4 months ago (April 1, 1997)
Published Online	28 years, 4 months ago (April 1, 1997)
Published Print	28 years, 4 months ago (April 1, 1997)

Funders 0

None

BibTeX

@article{Gerritsen_1997, title={CREB-binding protein/p300 are transcriptional coactivators of p65}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.7.2927}, DOI={10.1073/pnas.94.7.2927}, number={7}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Gerritsen, Mary E. and Williams, Amy J. and Neish, Andrew S. and Moore, Sarah and Shi, Yang and Collins, Tucker}, year={1997}, month=apr, pages={2927–2932} }

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