DOI: 10.1073/pnas.94.7.2838. Unwinding of the box I element of a herpes simplex virus type 1 origin by a complex of the viral origin binding protein, single-strand DNA binding protein, and single-stranded DNA

Unwinding of the box I element of a herpes simplex virus type 1 origin by a complex of the viral origin binding protein, single-strand DNA binding protein, and single-stranded DNA

10.1073/pnas.94.7.2838

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The herpes simplex virus type 1 (HSV-1) genome contains three origins of replication: ori L and two copies of ori S . These origins contain specific sequences, box I and box II, linked by an AT-rich segment, that are recognized by an HSV-1-encoded origin binding protein (UL9 protein) which also possesses DNA helicase activity. Despite its intrinsic helicase activity, the UL9 protein is unable to unwind ori S or the box I element of ori S , either in the presence or absence of the HSV-1-encoded single-strand DNA binding protein, ICP8. However, a complex of the UL9 protein and ICP8 can unwind box I if it contains a 3′ single-stranded tail at least 18 nt in length positioned downstream of box I. These findings suggest a model for the initiation of HSV-1 DNA replication in which a complex consisting of the UL9 protein bound to box I, and ICP8 bound to single-stranded DNA generated at the A+T rich linker, perhaps as a consequence of transcription, unwinds an HSV-1 origin of replication to provide access to the replication machinery with the consequent initiation of viral DNA replication. This mode of unwinding is distinct from that observed for other animal viruses—e.g., simian virus 40 or bovine papilloma virus—in which the initiator protein, T antigen, or E1 protein alone, unwinds elements of the origin sequence, and the single-strand DNA binding protein serves only to keep the separated strands apart.

Bibliography

Lee, S. S.-K., & Lehman, I. R. (1997). Unwinding of the box I element of a herpes simplex virus type 1 origin by a complex of the viral origin binding protein, single-strand DNA binding protein, and single-strandedâDNA. Proceedings of the National Academy of Sciences, 94(7), 2838â2842.

Authors 2

Sam S.-K. Lee (first)
I. R. Lehman (additional)

References 37 Referenced 43

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:28 p.m.)
Indexed	1 year, 3 months ago (May 17, 2024, 10:09 p.m.)
Issued	28 years, 4 months ago (April 1, 1997)
Published	28 years, 4 months ago (April 1, 1997)
Published Online	28 years, 4 months ago (April 1, 1997)
Published Print	28 years, 4 months ago (April 1, 1997)

Funders 0

None

BibTeX

@article{Lee_1997, title={Unwinding of the box I element of a herpes simplex virus type 1 origin by a complex of the viral origin binding protein, single-strand DNA binding protein, and single-stranded DNA}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.7.2838}, DOI={10.1073/pnas.94.7.2838}, number={7}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Lee, Sam S.-K. and Lehman, I. R.}, year={1997}, month=apr, pages={2838–2842} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        5,
        18
      ]
    ],
    "date-time": "2024-05-18T02:09:44Z",
    "timestamp": 1715998184166
  },
  "reference-count": 37,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "7",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1997,
        4
      ]
    ]
  },
  "abstract": "<jats:p>\n            The herpes simplex virus type 1 (HSV-1) genome contains three origins of replication: ori\n            <jats:sub>L</jats:sub>\n            and two copies of ori\n            <jats:sub>S</jats:sub>\n            . These origins contain specific sequences, box I and box II, linked by an AT-rich segment, that are recognized by an HSV-1-encoded origin binding protein (UL9 protein) which also possesses DNA helicase activity. Despite its intrinsic helicase activity, the UL9 protein is unable to unwind ori\n            <jats:sub>S</jats:sub>\n            or the box I element of ori\n            <jats:sub>S</jats:sub>\n            , either in the presence or absence of the HSV-1-encoded single-strand DNA binding protein, ICP8. However, a complex of the UL9 protein and ICP8 can unwind box I if it contains a 3\u2032 single-stranded tail at least 18 nt in length positioned downstream of box I. These findings suggest a model for the initiation of HSV-1 DNA replication in which a complex consisting of the UL9 protein bound to box I, and ICP8 bound to single-stranded DNA generated at the A+T rich linker, perhaps as a consequence of transcription, unwinds an HSV-1 origin of replication to provide access to the replication machinery with the consequent initiation of viral DNA replication. This mode of unwinding is distinct from that observed for other animal viruses\u2014e.g., simian virus 40 or bovine papilloma virus\u2014in which the initiator protein, T antigen, or E1 protein alone, unwinds elements of the origin sequence, and the single-strand DNA binding protein serves only to keep the separated strands apart.\n          </jats:p>",
  "DOI": "10.1073/pnas.94.7.2838",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        26
      ]
    ],
    "date-time": "2002-07-26T14:31:44Z",
    "timestamp": 1027693904000
  },
  "page": "2838-2842",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 43,
  "title": "Unwinding of the box I element of a herpes simplex virus type 1 origin by a complex of the viral origin binding protein, single-strand DNA binding protein, and single-stranded\u2009DNA",
  "prefix": "10.1073",
  "volume": "94",
  "author": [
    {
      "given": "Sam S.-K.",
      "family": "Lee",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Biochemistry, Stanford University, Beckman Center, Stanford, CA 94305-5307"
        }
      ]
    },
    {
      "given": "I. R.",
      "family": "Lehman",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemistry, Stanford University, Beckman Center, Stanford, CA 94305-5307"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1997,
        4
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_3_3_1_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.83.17.6322"
    },
    {
      "key": "e_1_3_3_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.85.15.5414"
    },
    {
      "key": "e_1_3_3_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.62.2.435-443.1988"
    },
    {
      "key": "e_1_3_3_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1099/0022-1317-73-2-313"
    },
    {
      "key": "e_1_3_3_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.58.070189.003323"
    },
    {
      "key": "e_1_3_3_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)52296-1"
    },
    {
      "key": "e_1_3_3_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.66.7.3986-3995.1992"
    },
    {
      "key": "e_1_3_3_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(17)44884-8"
    },
    {
      "key": "e_1_3_3_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)49713-5"
    },
    {
      "key": "e_1_3_3_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mcb.8.10.4018-4027.1988"
    },
    {
      "key": "e_1_3_3_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.68.6.3841-3849.1994"
    },
    {
      "key": "e_1_3_3_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.63.3.1275-1283.1989"
    },
    {
      "key": "e_1_3_3_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.67.12.7680-7683.1993"
    },
    {
      "key": "e_1_3_3_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.66.11.6735-6746.1992"
    },
    {
      "key": "e_1_3_3_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.68.7.4126-4136.1994"
    },
    {
      "key": "e_1_3_3_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)54062-X"
    },
    {
      "key": "e_1_3_3_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)54063-1"
    },
    {
      "key": "e_1_3_3_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.cb.05.110189.001213"
    },
    {
      "key": "e_1_3_3_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(90)90730-3"
    },
    {
      "key": "e_1_3_3_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(88)90102-X"
    },
    {
      "key": "e_1_3_3_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1996.tb00520.x"
    },
    {
      "key": "e_1_3_3_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.65.6.3284-3292.1991"
    },
    {
      "key": "e_1_3_3_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.67.2.711-715.1993"
    },
    {
      "key": "e_1_3_3_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.84.1.16"
    },
    {
      "key": "e_1_3_3_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)54274-5"
    },
    {
      "key": "e_1_3_3_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.86.24.9757"
    },
    {
      "key": "e_1_3_3_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.90.18.8444"
    },
    {
      "key": "e_1_3_3_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1099/0022-1317-74-7-1349"
    },
    {
      "key": "e_1_3_3_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)62048-X"
    },
    {
      "key": "e_1_3_3_30_2",
      "first-page": "4242",
      "volume": "262",
      "author": "O\u2019Donnell M E",
      "year": "1987",
      "unstructured": "M E O\u2019Donnell, P Elias, I R Lehman J Biol Chem 262, 4242\u20134259 (1987).",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "e_1_3_3_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.270.32.19028"
    },
    {
      "key": "e_1_3_3_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.91.11.4629"
    },
    {
      "key": "e_1_3_3_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.65.5.2601-2611.1991"
    },
    {
      "key": "e_1_3_3_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.65.6.3140-3150.1991"
    },
    {
      "key": "e_1_3_3_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.85.22.8454"
    },
    {
      "key": "e_1_3_3_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(88)90014-1"
    },
    {
      "key": "e_1_3_3_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.62.070193.000333"
    }
  ],
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.94.7.2838",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T19:28:50Z",
    "timestamp": 1649878130000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.94.7.2838"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1997,
        4
      ]
    ]
  },
  "references-count": 37,
  "journal-issue": {
    "issue": "7",
    "published-print": {
      "date-parts": [
        [
          1997,
          4
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.94.7.2838"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.94.7.2838",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1997,
        4
      ]
    ]
  },
  "assertion": [
    {
      "value": "1997-01-13",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "1997-04-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}