DOI: 10.1073/pnas.94.6.2238. Molecular characterization of human and mouse fatty acid amide hydrolases

Molecular characterization of human and mouse fatty acid amide hydrolases

10.1073/pnas.94.6.2238

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Recently, we reported the isolation, cloning, and expression of a rat enzyme, fatty acid amide hydrolase (FAAH), that degrades bioactive fatty acid amides like oleamide and anandamide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Here, we report the molecular characterization of both a mouse and a human FAAH and compare these enzymes to the rat FAAH. The enzymes are well conserved in primary structure, with the mouse and rat FAAHs sharing 91% amino acid identity and the human FAAH sharing 82% and 84% identity with the rat FAAH and mouse FAAH, respectively. In addition, the expressed human and rat FAAHs behave biochemically as membrane proteins of comparable molecular size and show similar, but distinguishable, enzymological properties. The identification of highly homologous FAAH proteins in rat, mouse, and human supports a general role for the fatty acid amides in mammalian biology.

Bibliography

Giang, D. K., & Cravatt, B. F. (1997). Molecular characterization of human and mouse fatty acidâamideâhydrolases. Proceedings of the National Academy of Sciences, 94(6), 2238â2242.

Authors 2

Dan K. Giang (first)
Benjamin F. Cravatt (additional)

References 27 Referenced 350

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 2 months ago (June 6, 2022, 9:43 p.m.)
Indexed	1 day, 20 hours ago (Aug. 26, 2025, 3:15 a.m.)
Issued	28 years, 5 months ago (March 18, 1997)
Published	28 years, 5 months ago (March 18, 1997)
Published Online	28 years, 5 months ago (March 18, 1997)
Published Print	28 years, 5 months ago (March 18, 1997)

Funders 0

None

BibTeX

@article{Giang_1997, title={Molecular characterization of human and mouse fatty acid amide hydrolases}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.6.2238}, DOI={10.1073/pnas.94.6.2238}, number={6}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Giang, Dan K. and Cravatt, Benjamin F.}, year={1997}, month=mar, pages={2238–2242} }

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