DOI: 10.1073/pnas.94.6.2145. From phosphatases to vanadium peroxidases: A similar architecture of the active site

From phosphatases to vanadium peroxidases: A similar architecture of the active site

10.1073/pnas.94.6.2145

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We show here that the amino acid residues contributing to the active sites of the vanadate containing haloperoxidases are conserved within three families of acid phosphatases; this suggests that the active sites of these enzymes are very similar. This is confirmed by activity measurements showing that apochloroperoxidase exhibits phosphatase activity. These observations not only reveal interesting evolutionary relationships between these groups of enzymes but may also have important implications for the research on acid phosphatases, especially glucose-6-phosphatase—the enzyme affected in von Gierke disease—of which the predicted membrane topology may have to be reconsidered.

Bibliography

Hemrika, W., Renirie, R., Dekker, H. L., Barnett, P., & Wever, R. (1997). From phosphatases to vanadium peroxidases: A similar architecture of the activeâsite. Proceedings of the National Academy of Sciences, 94(6), 2145â2149.

Authors 5

Wieger Hemrika (first)
Rokus Renirie (additional)
Henk L. Dekker (additional)
Phil Barnett (additional)
Ron Wever (additional)

References 37 Referenced 143

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:32 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:17 p.m.)
Indexed	1 week, 1 day ago (Aug. 28, 2025, 7:58 a.m.)
Issued	28 years, 5 months ago (March 18, 1997)
Published	28 years, 5 months ago (March 18, 1997)
Published Online	28 years, 5 months ago (March 18, 1997)
Published Print	28 years, 5 months ago (March 18, 1997)

Funders 0

None

BibTeX

@article{Hemrika_1997, title={From phosphatases to vanadium peroxidases: A similar architecture of the active site}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.6.2145}, DOI={10.1073/pnas.94.6.2145}, number={6}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Hemrika, Wieger and Renirie, Rokus and Dekker, Henk L. and Barnett, Phil and Wever, Ron}, year={1997}, month=mar, pages={2145–2149} }

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