DOI: 10.1073/pnas.94.5.1785. Direct<i>in vivo</i>measurement of targeted binding in a human tumor xenograft

Directin vivomeasurement of targeted binding in a human tumor xenograft

10.1073/pnas.94.5.1785

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Binding is crucial to the function of most biologically active molecules, but difficult to quantify directly in living tissue. To this end, fluorescence recovery after photobleaching was used to detect the immobilization of fluorescently labeled ligand caused by binding to receptorsin vivo. Measurements of mAb affinity to target antigen within human tumor xenografts revealed a saturable binding isotherm, from which anin vivocarcinoembryonic antigen density of 0.56 nmol/g (5.0 × 105/cell) and an association constant ofKa⩽ 4 × 107M−1were estimated. The present method can be adapted forin vivostudies of cell signaling, targeted drugs, gene therapy, and other processes involving receptor-ligand binding.

Bibliography

Berk, D. A., Yuan, F., Leunig, M., & Jain, R. K. (1997). Directin vivomeasurement of targeted binding in a human tumorâxenograft. Proceedings of the National Academy of Sciences, 94(5), 1785â1790.

Authors 4

David A. Berk (first)
Fan Yuan (additional)
Michael Leunig (additional)
Rakesh K. Jain (additional)

References 35 Referenced 114

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Dates

Type	When
Created	23 years ago (July 26, 2002, 10:31 a.m.)
Deposited	1 year, 7 months ago (Jan. 3, 2024, 11:45 p.m.)
Indexed	3 weeks, 1 day ago (Aug. 2, 2025, 12:55 a.m.)
Issued	28 years, 5 months ago (March 4, 1997)
Published	28 years, 5 months ago (March 4, 1997)
Published Online	28 years, 5 months ago (March 4, 1997)
Published Print	28 years, 5 months ago (March 4, 1997)

Funders 0

None

BibTeX

@article{Berk_1997, title={Directin vivomeasurement of targeted binding in a human tumor xenograft}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.5.1785}, DOI={10.1073/pnas.94.5.1785}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Berk, David A. and Yuan, Fan and Leunig, Michael and Jain, Rakesh K.}, year={1997}, month=mar, pages={1785–1790} }

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