DOI: 10.1073/pnas.94.4.1568. Stabilization of ion selectivity filter by pore loop ion pairs in an inwardly rectifying potassium channel

Stabilization of ion selectivity filter by pore loop ion pairs in an inwardly rectifying potassium channel

10.1073/pnas.94.4.1568

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Ion selectivity is critical for the biological functions of voltage-dependent cation channels and is achieved by specific ion binding to a pore region called the selectivity filter. In voltage-gated K+, Na+and Ca2+channels, the selectivity filter is formed by a short polypeptide loop (called the H5 or P region) between the fifth and sixth transmembrane segments, donated by each of the four subunits or internal homologous domains forming the channel. While mutagenesis studies on voltage-gated K+channels have begun to shed light on the structural organization of this pore region, little is known about the physical and chemical interactions that maintain the structural stability of the selectivity filter. Here we show that in an inwardly rectifying K+(IRK) channel, IRK1, short range interactions of an ion pair in the H5 pore loop are crucial for pore structure and ion permeation. The two residues, a glutamate and an arginine, appear to form exposed salt bridges in the tetrameric channel. Alteration or disruption of such ion pair interactions dramatically alters ion selectivity and permeation. Since this ion pair is conserved in all IRK channels, it may constitute a general mechanism for maintaining the stability of the pore structure in this channel superfamily.

Bibliography

Yang, J., Yu, M., Jan, Y. N., & Jan, L. Y. (1997). Stabilization of ion selectivity filter by pore loop ion pairs in an inwardly rectifying potassiumâchannel. Proceedings of the National Academy of Sciences, 94(4), 1568â1572.

Authors 4

Jian Yang (first)
Mei Yu (additional)
Yuh Nung Jan (additional)
Lily Yeh Jan (additional)

References 28 Referenced 92

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:40 a.m.)
Deposited	1 year, 7 months ago (Jan. 4, 2024, 12:05 a.m.)
Indexed	4 months ago (April 28, 2025, 5:01 p.m.)
Issued	28 years, 6 months ago (Feb. 18, 1997)
Published	28 years, 6 months ago (Feb. 18, 1997)
Published Online	28 years, 6 months ago (Feb. 18, 1997)
Published Print	28 years, 6 months ago (Feb. 18, 1997)

Funders 0

None

BibTeX

@article{Yang_1997, title={Stabilization of ion selectivity filter by pore loop ion pairs in an inwardly rectifying potassium channel}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.4.1568}, DOI={10.1073/pnas.94.4.1568}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Yang, Jian and Yu, Mei and Jan, Yuh Nung and Jan, Lily Yeh}, year={1997}, month=feb, pages={1568–1572} }

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