DOI: 10.1073/pnas.94.4.1074. Nuclear integration of JAK/STAT and Ras/AP-1 signaling by CBP and p300

Nuclear integration of JAK/STAT and Ras/AP-1 signaling by CBP and p300

10.1073/pnas.94.4.1074

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We report that interferon γ (IFN-γ) inhibits transcription of the macrophage scavenger receptor gene by antagonizing the Ras-dependent activities of AP-1 and cooperating ets domain transcription factors, apparently as a result of competition between AP-1/ets factors and activated STAT1 for limiting amounts of CBP and p300. Consistent with this model, STAT1α interacts directly with CBP in cells, and microinjection of anti-CBP and anti-p300 antibodies blocks transcriptional responses to IFN-γ. Cells lacking STAT1 fail to inhibit AP-1/ets activity, and overexpression of CBP both potentiates IFN-γ-dependent transcription and relieves AP-1/ets repression. Thus, CBP and p300 integrate both positive and negative effects of IFN-γ on gene expression by serving as essential coactivators of STAT1α, modulating gene-specific responses to simultaneous activation of two or more signal transduction pathways.

Bibliography

Horvai, A. E., Xu, L., Korzus, E., Brard, G., Kalafus, D., Mullen, T.-M., Rose, D. W., Rosenfeld, M. G., & Glass, C. K. (1997). Nuclear integration of JAK/STAT and Ras/AP-1 signaling by CBPâandâp300. Proceedings of the National Academy of Sciences, 94(4), 1074â1079.

Authors 9

Andrew E. Horvai (first)
Lan Xu (additional)
Edward Korzus (additional)
Gyan Brard (additional)
Daniel Kalafus (additional)
Tina-Marie Mullen (additional)
David W. Rose (additional)
Michael G. Rosenfeld (additional)
Christopher K. Glass (additional)

References 37 Referenced 345

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:43 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:09 p.m.)
Indexed	2 months ago (June 27, 2025, 9:44 a.m.)
Issued	28 years, 6 months ago (Feb. 18, 1997)
Published	28 years, 6 months ago (Feb. 18, 1997)
Published Online	28 years, 6 months ago (Feb. 18, 1997)
Published Print	28 years, 6 months ago (Feb. 18, 1997)

Funders 0

None

BibTeX

@article{Horvai_1997, title={Nuclear integration of JAK/STAT and Ras/AP-1 signaling by CBP and p300}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.4.1074}, DOI={10.1073/pnas.94.4.1074}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Horvai, Andrew E. and Xu, Lan and Korzus, Edward and Brard, Gyan and Kalafus, Daniel and Mullen, Tina-Marie and Rose, David W. and Rosenfeld, Michael G. and Glass, Christopher K.}, year={1997}, month=feb, pages={1074–1079} }

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