DOI: 10.1073/pnas.94.3.901. An α- <i>E-catenin</i> gene trap mutation defines its function in preimplantation development

An α- E-catenin gene trap mutation defines its function in preimplantation development

10.1073/pnas.94.3.901

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Catenins are proteins associated with the cytoplasmic domain of cadherins, a family of transmembrane cell adhesion molecules. The cadherin–catenin adhesion system is involved in morphogenesis during development and in the maintenance of the integrity of different tissue types. Using a gene trap strategy, we have isolated a mouse mutation for the gene encoding the α-E-catenin. This form of the α-catenin appears frequently coexpressed with E-cadherin in epithelial cell types. The mutation obtained eliminates the carboxyl-terminal third of the protein but nevertheless provokes a complete loss-of-function phenotype. Homozygous mutants show disruption of the trophoblast epithelium (the first differentiated embryonic tissue), and development is consequently blocked at the blastocyst stage. This phenotype parallels the defects observed in E-cadherin mutant embryos. Our results show the requirement of the α-E-catenin carboxy terminus for its function and represent evidence of the role of the α-E-catenin in vivo , identifying this molecule as the natural partner of the E-cadherin in trophoblast epithelium.

Bibliography

Torres, M., Stoykova, A., Huber, O., Chowdhury, K., Bonaldo, P., Mansouri, A., Butz, S., Kemler, R., & Gruss, P. (1997). An Î±- E-catenin gene trap mutation defines its function in preimplantationâdevelopment. Proceedings of the National Academy of Sciences, 94(3), 901â906.

Authors 9

Miguel Torres (first)
Anastassia Stoykova (additional)
Otmar Huber (additional)
Kamal Chowdhury (additional)
Paolo Bonaldo (additional)
Ahmed Mansouri (additional)
Stefan Butz (additional)
Rolf Kemler (additional)
Peter Gruss (additional)

References 34 Referenced 181

10.1126/science.2006419
10.1016/0168-9525(93)90250-L
10.1002/j.1460-2075.1989.tb03563.x
10.1091/mbc.1.1.37
10.1016/0014-5793(94)01205-9
10.1083/jcb.125.6.1327
10.1083/jcb.125.6.1341
10.1242/jcs.107.12.3655
10.1073/pnas.92.19.8813
10.1083/jcb.130.1.67
10.1091/mbc.2.4.261
10.1073/pnas.88.20.9156
10.1016/0092-8674(91)90392-C
10.1016/0092-8674(92)90103-J
10.1126/science.1962194
10.1126/science.257.5073.1142.b
W W Franke, M D Goldsmith, R Zimbelmann, H M Mueller, D L Schiller, P Cowin Biochemistry 86, 4027–4031 (1989). / Biochemistry by Franke W W (1989)
10.1083/jcb.127.1.235
10.1006/dbio.1994.1124
10.1073/pnas.91.17.8263
10.1073/pnas.92.3.855
10.1126/science.2497519
10.1101/gad.5.9.1513
10.1101/gad.6.6.903
10.1073/pnas.92.14.6592
A Nagy, J Rossant Gene Targeting: A Practical Approach, ed A Joyner (IRL, Oxford, U.K.), pp. 147–179 (1993). / Gene Targeting: A Practical Approach by Nagy A (1993)
10.1016/B978-0-12-164717-9.50020-9
A Gossler, J Zachgo Gene Targeting: A Practical Approach, ed A Joyner (IRL, Oxford, U.K.), pp. 147–179 (1993). / Gene Targeting: A Practical Approach by Gossler A (1993)
10.1242/jcs.1993.Supplement_17.21
10.1111/j.1440-169X.1994.00059.x
10.1016/0012-1606(87)90498-2
10.1242/dev.121.11.3529
10.1016/0968-0004(94)90057-4
10.1083/jcb.123.2.477

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:03 p.m.)
Indexed	4 weeks ago (Aug. 7, 2025, 4:41 p.m.)
Issued	28 years, 7 months ago (Feb. 4, 1997)
Published	28 years, 7 months ago (Feb. 4, 1997)
Published Online	28 years, 7 months ago (Feb. 4, 1997)
Published Print	28 years, 7 months ago (Feb. 4, 1997)

Funders 0

None

BibTeX

@article{Torres_1997, title={An α- E-catenin gene trap mutation defines its function in preimplantation development}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.3.901}, DOI={10.1073/pnas.94.3.901}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Torres, Miguel and Stoykova, Anastassia and Huber, Otmar and Chowdhury, Kamal and Bonaldo, Paolo and Mansouri, Ahmed and Butz, Stefan and Kemler, Rolf and Gruss, Peter}, year={1997}, month=feb, pages={901–906} }

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