DOI: 10.1073/pnas.94.3.1035. <i>Arabidopsis thaliana CBF1</i> encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit

Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit

10.1073/pnas.94.3.1035

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Recent efforts have defined a cis-acting DNA regulatory element in plants, the C-repeat/dehydration responsive element (DRE), that stimulates transcription in response to low temperature and water deficit. Here we report the isolation of an Arabidopsis thaliana cDNA that encodes a C-repeat/DRE binding factor, CBF1 ( C -repeat/DRE B inding F actor 1 ). Analysis of the deduced CBF1 amino acid sequence indicates that the protein has a molecular mass of 24 kDa, a potential nuclear localization sequence, and a possible acidic activation domain. CBF1 also has an AP2 domain, which is a DNA-binding motif of about 60 aa present in the Arabidopsis proteins APETALA2, AINTEGUMENTA, and TINY; the tobacco ethylene response element binding proteins; and numerous other plant proteins of unknown function. The transcript levels for CBF1 , which appears to be a single or low copy number gene, did not change appreciably in plants exposed to low temperature or in detached leaves subjected to water deficit. Binding of CBF1 to the C-repeat/DRE was demonstrated in gel shift assays using recombinant CBF1 protein expressed in Escherichia coli . Moreover, expression of CBF1 in yeast was found to activate transcription of reporter genes containing the C-repeat/DRE as an upstream activator sequence but not mutant versions of the DNA element. We conclude that CBF1 can function as a transcriptional activator that binds to the C-repeat/DRE DNA regulatory element and, thus, is likely to have a role in cold- and dehydration-regulated gene expression in Arabidopsis .

Bibliography

Stockinger, E. J., Gilmour, S. J., & Thomashow, M. F. (1997). Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature andâwaterâdeficit. Proceedings of the National Academy of Sciences, 94(3), 1035â1040.

Authors 3

Eric J. Stockinger (first)
Sarah J. Gilmour (additional)
Michael F. Thomashow (additional)

References 37 Referenced 1,452

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:43 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:03 p.m.)
Indexed	2 days, 2 hours ago (Aug. 30, 2025, 12:49 p.m.)
Issued	28 years, 6 months ago (Feb. 4, 1997)
Published	28 years, 6 months ago (Feb. 4, 1997)
Published Online	28 years, 6 months ago (Feb. 4, 1997)
Published Print	28 years, 6 months ago (Feb. 4, 1997)

Funders 0

None

BibTeX

@article{Stockinger_1997, title={Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.3.1035}, DOI={10.1073/pnas.94.3.1035}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Stockinger, Eric J. and Gilmour, Sarah J. and Thomashow, Michael F.}, year={1997}, month=feb, pages={1035–1040} }

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