DOI: 10.1073/pnas.94.26.14602. Protamine-Cre recombinase transgenes efficiently recombine target sequences in the male germ line of mice, but not in embryonic stem cells

Protamine-Cre recombinase transgenes efficiently recombine target sequences in the male germ line of mice, but not in embryonic stem cells

10.1073/pnas.94.26.14602

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The production of subtle or conditional mutations in mice through the combined use of site-specific and homologous recombination has become an increasingly widespread experimental paradigm in mammalian genetics. Embryonic stem cells containing recombinase transgenes that were expressed in the male germ line, but not in other tissues or in the embryonic stem cells themselves, would substantially simplify the production of such alleles. Here we show that transgenes comprised of the mouse protamine 1 promoter and the Cre recombinase coding sequence mediate the efficient recombination of a Cre target transgene in the male germ line, but not in other tissues. Embryonic stem cell lines generated from one of these transgenic strains were transfected with targeting vectors that included loxP-flanked selectable markers, and homologously recombined alleles containing the marker and functional loxP sites were isolated. These results establish the potential of the system for substantially reducing the time, effort, and resources required to produce homologously recombined alleles in mice that have been secondarily rearranged by a site-specific recombinase.

Bibliography

OâGorman, S., Dagenais, N. A., Qian, M., & Marchuk, Y. (1997). Protamine-Cre recombinase transgenes efficiently recombine target sequences in the male germ line of mice, but not in embryonic stemâcells. Proceedings of the National Academy of Sciences, 94(26), 14602â14607.

Authors 4

Stephen O’Gorman (first)
Nicole A. Dagenais (additional)
Min Qian (additional)
Yelena Marchuk (additional)

References 45 Referenced 382

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:35 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:27 p.m.)
Indexed	4 months, 3 weeks ago (March 30, 2025, 7:03 p.m.)
Issued	27 years, 8 months ago (Dec. 23, 1997)
Published	27 years, 8 months ago (Dec. 23, 1997)
Published Online	27 years, 8 months ago (Dec. 23, 1997)
Published Print	27 years, 8 months ago (Dec. 23, 1997)

Funders 0

None

BibTeX

@article{O_Gorman_1997, title={Protamine-Cre recombinase transgenes efficiently recombine target sequences in the male germ line of mice, but not in embryonic stem cells}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.26.14602}, DOI={10.1073/pnas.94.26.14602}, number={26}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={O’Gorman, Stephen and Dagenais, Nicole A. and Qian, Min and Marchuk, Yelena}, year={1997}, month=dec, pages={14602–14607} }

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