DOI: 10.1073/pnas.94.26.14383. Archaeal-type lysyl-tRNA synthetase in the Lyme disease spirochete <i>Borrelia burgdorferi</i>

Archaeal-type lysyl-tRNA synthetase in the Lyme disease spirochete Borrelia burgdorferi

10.1073/pnas.94.26.14383

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Lysyl-tRNAs are essential for protein biosynthesis by ribosomal mRNA translation in all organisms. They are synthesized by lysyl-tRNA synthetases (EC 6.1.1.6 ), a group of enzymes composed of two unrelated families. In bacteria and eukarya, all known lysyl-tRNA synthetases are subclass IIc-type aminoacyl-tRNA synthetases, whereas some archaea have been shown to contain an unrelated class I-type lysyl-tRNA synthetase. Examination of the preliminary genomic sequence of the bacterial pathogen Borrelia burgdorferi , the causative agent of Lyme disease, indicated the presence of an open reading frame with over 55% similarity at the amino acid level to archaeal class I-type lysyl-tRNA synthetases. In contrast, no coding region with significant similarity to any class II-type lysyl-tRNA synthetase could be detected. Heterologous expression of this open reading frame in Escherichia coli led to the production of a protein with canonical lysyl-tRNA synthetase activity in vitro . Analysis of B. burgdorferi mRNA showed that the lysyl-tRNA synthetase-encoding gene is highly expressed, confirming that B. burgdorferi contains a functional class I-type lysyl-tRNA synthetase. The detection of an archaeal-type lysyl-tRNA synthetase in B. burgdorferi and other pathogenic spirochetes, but not to date elsewhere in bacteria or eukarya, indicates that the gene that encodes this enzyme has a common origin with its orthologue from the archaeal kingdom. This difference between the lysyl-tRNA synthetases of spirochetes and their hosts may be readily exploitable for the development of anti-spirochete therapeutics.

Bibliography

Ibba, M., Bono, J. L., Rosa, P. A., & SÃ¶ll, D. (1997). Archaeal-type lysyl-tRNA synthetase in the Lyme disease spirochete Borreliaâburgdorferi. Proceedings of the National Academy of Sciences, 94(26), 14383â14388.

Authors 4

Michael Ibba (first)
James L. Bono (additional)
Patricia A. Rosa (additional)
Dieter Söll (additional)

References 40 Referenced 66

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:33 p.m.)
Indexed	1 month ago (July 30, 2025, 10:01 a.m.)
Issued	27 years, 8 months ago (Dec. 23, 1997)
Published	27 years, 8 months ago (Dec. 23, 1997)
Published Online	27 years, 8 months ago (Dec. 23, 1997)
Published Print	27 years, 8 months ago (Dec. 23, 1997)

Funders 0

None

BibTeX

@article{Ibba_1997, title={Archaeal-type lysyl-tRNA synthetase in the Lyme disease spirochete Borrelia burgdorferi}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.26.14383}, DOI={10.1073/pnas.94.26.14383}, number={26}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Ibba, Michael and Bono, James L. and Rosa, Patricia A. and Söll, Dieter}, year={1997}, month=dec, pages={14383–14388} }

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