DOI: 10.1073/pnas.94.26.14291. Visualization of a peripheral stalk in V-type ATPase: Evidence for the stator structure essential to rotational catalysis

Visualization of a peripheral stalk in V-type ATPase: Evidence for the stator structure essential to rotational catalysis

10.1073/pnas.94.26.14291

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

F- and V-type ATPases are central enzymes in energy metabolism that couple synthesis or hydrolysis of ATP to the translocation of H + or Na + across biological membranes. They consist of a soluble headpiece that contains the catalytic sites and an integral membrane-bound part that conducts the ion flow. Energy coupling is thought to occur through the physical rotation of a stalk that connects the two parts of the enzyme complex. This mechanism implies that a stator-like structure prevents the rotation of the headpiece relative to the membrane-bound part. Such a structure has not been observed to date. Here, we report the projected structure of the V-type Na + -ATPase of Clostridium fervidus as determined by electron microscopy. Besides the central stalk, a second stalk of 130 Å in length is observed that connects the headpiece and membrane-bound part in the periphery of the complex. This additional stalk is likely to be the stator.

Bibliography

Boekema, E. J., Ubbink-Kok, T., Lolkema, J. S., Brisson, A., & Konings, W. N. (1997). Visualization of a peripheral stalk in V-type ATPase: Evidence for the stator structure essential to rotationalâcatalysis. Proceedings of the National Academy of Sciences, 94(26), 14291â14293.

Authors 5

E. J. Boekema (first)
T. Ubbink-Kok (additional)
J. S. Lolkema (additional)
A. Brisson (additional)
W. N. Konings (additional)

References 25 Referenced 82

10.1016/S0959-440X(96)80114-X
10.1038/370621a0
10.1016/0005-2728(93)90063-L
10.1038/381623a0
10.1038/386299a0
10.1002/bbpc.19961001215
10.1007/BF02113984
10.1128/jb.179.4.1274-1279.1997
10.1016/S0076-6879(88)64033-X
10.1016/S0005-2728(05)80328-7
M Van Heel, J Frank Ultramicroscopy 6, 187–194 (1981). / Ultramicroscopy by Van Heel M (1981)
10.1073/pnas.90.17.7975
10.1515/znc-1988-3-412
10.1021/bi00474a019
10.1038/nsb1094-660
10.1016/S0021-9258(19)37029-2
10.1111/j.1438-8677.1995.tb00826.x
10.1093/protein/5.1.7
10.1016/S0014-5793(96)01127-1
10.1111/j.1432-1033.1995.tb20534.x
10.1242/jeb.199.5.1147
10.1016/0304-4173(84)90016-8
10.1002/bbpc.198800257
10.1073/pnas.92.24.10964
10.1038/nsb0397-198

Dates

Type	When
Created	23 years ago (July 26, 2002, 10:35 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:26 p.m.)
Indexed	2 weeks, 3 days ago (Aug. 6, 2025, 9:24 a.m.)
Issued	27 years, 8 months ago (Dec. 23, 1997)
Published	27 years, 8 months ago (Dec. 23, 1997)
Published Online	27 years, 8 months ago (Dec. 23, 1997)
Published Print	27 years, 8 months ago (Dec. 23, 1997)

Funders 0

None

BibTeX

@article{Boekema_1997, title={Visualization of a peripheral stalk in V-type ATPase: Evidence for the stator structure essential to rotational catalysis}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.26.14291}, DOI={10.1073/pnas.94.26.14291}, number={26}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Boekema, E. J. and Ubbink-Kok, T. and Lolkema, J. S. and Brisson, A. and Konings, W. N.}, year={1997}, month=dec, pages={14291–14293} }

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